Any feedback?
Literature summary for 3.1.1.81 extracted from
- Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia (2015), Sci. Rep., 5, 8372 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, X-ray structures of the enzyme bound to a fatty acid and to its cognate substrate N-decanoyl-L-homoserine lactone. The structures highlight possible structural determinants that may be involved in its extreme thermal stability | Vulcanisaeta moutnovskia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0021 | - |
gamma-dodecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia |  |
| 0.047 | - |
gamma-nonalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 0.105 | - |
delta-undecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 0.12 | - |
gamma-undecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 0.186 | - |
N-3-oxooctanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 0.231 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 0.262 | - |
N-octanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 0.839 | - |
delta-dodecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 0.872 | - |
gamma-heptalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 1.22 | - |
delta-nonalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.709 | - |
Gamma-caprolactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | bi-cobalt metalloenzyme. The two metal cations are coordinated by four histidines, an aspartic acid and a carboxylated lysine residue. Both of the metal cations are bridged by a putatively catalytic water molecule | Vulcanisaeta moutnovskia | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vulcanisaeta moutnovskia | F0QXN6 | - |
- |
| Vulcanisaeta moutnovskia 768-28 | F0QXN6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vulcanisaeta moutnovskia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| delta-dodecalactone + H2O | - |
Vulcanisaeta moutnovskia | 5-hydroxydodecanoic acid | - |
? | |
| delta-nonalactone + H2O | - |
Vulcanisaeta moutnovskia | 5-hydroxynonanoic acid | - |
? | |
| delta-nonalactone + H2O | - |
Vulcanisaeta moutnovskia 768-28 | 5-hydroxynonanoic acid | - |
? | |
| delta-undecalactone + H2O | - |
Vulcanisaeta moutnovskia | 5-hydroxyundecanoic acid | - |
? | |
| gamma-caprolactone + H2O | - |
Vulcanisaeta moutnovskia | 4-hydroxyhexanoic acid | - |
? | |
| gamma-dodecalactone + H2O | - |
Vulcanisaeta moutnovskia | 4-hydroxydodecanoic acid | - |
? | |
| gamma-heptalactone + H2O | - |
Vulcanisaeta moutnovskia | 4-hydroxyheptanoic acid | - |
? | |
| gamma-nonalactone + H2O | - |
Vulcanisaeta moutnovskia | 4-hydroxynonanoic acid | - |
? | |
| gamma-nonalactone + H2O | - |
Vulcanisaeta moutnovskia 768-28 | 4-hydroxynonanoic acid | - |
? | |
| gamma-undecalactone + H2O | - |
Vulcanisaeta moutnovskia | 4-hydroxyundecanoic acid | - |
? | |
| additional information | VmoLac is a proficient lactonase with promiscuous, poor esterase and phosphotriesterase activities | Vulcanisaeta moutnovskia | ? | - |
? | |
| additional information | VmoLac is a proficient lactonase with promiscuous, poor esterase and phosphotriesterase activities | Vulcanisaeta moutnovskia 768-28 | ? | - |
? | |
| N-3-oxooctanoyl-L-homoserine lactone + H2O | - |
Vulcanisaeta moutnovskia | N-3-oxooctanoyl-L-homoserine | - |
? | |
| N-3-oxooctanoyl-L-homoserine lactone + H2O | - |
Vulcanisaeta moutnovskia 768-28 | N-3-oxooctanoyl-L-homoserine | - |
? | |
| N-decanoyl-L-homoserine lactone + H2O | - |
Vulcanisaeta moutnovskia | N-decanoyl-L-homoserine | - |
? | |
| N-decanoyl-L-homoserine lactone + H2O | - |
Vulcanisaeta moutnovskia 768-28 | N-decanoyl-L-homoserine | - |
? | |
| N-octanoyl-L-homoserine lactone + H2O | - |
Vulcanisaeta moutnovskia | N-octanoyl-L-homoserine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| VmoLac | - |
Vulcanisaeta moutnovskia |
| VMUT_2255 | - |
Vulcanisaeta moutnovskia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 128 | - |
Tm-value | Vulcanisaeta moutnovskia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
gamma-dodecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 0.35 | - |
N-3-oxooctanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 0.47 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 0.59 | - |
N-octanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 8.86 | - |
gamma-undecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 27.25 | - |
gamma-heptalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 28.08 | - |
delta-dodecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 44.49 | - |
gamma-nonalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 60.78 | - |
delta-undecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 88.91 | - |
delta-nonalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 112.3 | - |
Gamma-caprolactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.88 | - |
N-3-oxooctanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 2.23 | - |
N-octanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 30.3 | - |
Gamma-caprolactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 31.1 | - |
gamma-heptalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 72.9 | - |
delta-nonalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 73.6 | - |
gamma-undecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 77.7 | - |
gamma-dodecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 335 | - |
delta-dodecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 580 | - |
delta-undecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 948 | - |
gamma-nonalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
