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Literature summary for 3.1.1.81 extracted from

  • Fan, X.; Liang, M.; Wang, L.; Chen, R.; Li, H.; Liu, X.
    Aii810, a novel cold-adapted N-acylhomoserine lactonase discovered in a metagenome, can strongly attenuate Pseudomonas aeruginosa virulence factors and biofilm formation (2017), Front. Microbiol., 8, 1950 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Application

Application Comment Organism
medicine the pathogen Pseudomonas aeruginosa uses quorum sensing to control virulence and biofilm formation. Enzymatic disruption of quorum sensing is a promising antiinfection therapeutic strategy that does not rely on antibiotics. Aii810, a cold-adapted N-acylhomoserine lactonase can attenuate Pseudomonas aeruginosa virulence factors and biofilm formation. It is an attractive enzyme for use as a therapeutic agent against Pseudomonas aeruginosa infection uncultured bacterium
pharmacology the pathogen Pseudomonas aeruginosa uses quorum sensing to control virulence and biofilm formation. Enzymatic disruption of quorum sensing is a promising antiinfection therapeutic strategy that does not rely on antibiotics. Aii810, a cold-adapted N-acylhomoserine lactonase can attenuate Pseudomonas aeruginosa virulence factors and biofilm formation. It is an attractive enzyme for use as a therapeutic agent against Pseudomonas aeruginosa infection uncultured bacterium

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29600
-
 calculated from sequence uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium A0A286S6I1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 uncultured bacterium N-3-oxododecanoyl-L-homoserine
-
 ?
N-butyryl-L-homoserine lactone + H2O
-
 uncultured bacterium N-butyryl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
Aii810
-
 uncultured bacterium
cold-adapted N-acylhomoserine lactonase
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 with 4-nitrophenyl acetate as a substrate uncultured bacterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 40 the enzyme maintains more than 50% of its maximal activity at 0-40°C uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable below uncultured bacterium
45
-
 12 h, strong decrease in activity uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 uncultured bacterium

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 the enzyme retains more than 70% activity in the pH range 7.0-9.5 but the activity abruptly falls after pH 6.5 uncultured bacterium

pI Value

Organism Comment pI Value Maximum pI Value
uncultured bacterium calculated from sequence
-
 6.86