Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.81 extracted from

  • Shastry, R.P.; Dolan, S.K.; Abdelhamid, Y.; Vittal, R.R.; Welch, M.
    Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66 (2018), FEMS Microbiol. Lett., 365, fny054.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
His6-tagged AiiE is overexpressed from pET-19m in Rosetta DE3 cells Enterobacter sp. CS66

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.01
-
 N-butanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
7.88
-
 N-hexanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
10.35
-
 N-octanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
14.88
-
 N-decanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
15.21
-
 N-3-oxododecanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 SDS-PAGE Enterobacter sp. CS66

Organism

Organism UniProt Comment Textmining
Enterobacter sp. CS66
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterobacter sp. CS66

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Enterobacter sp. CS66 N-3-oxododecanoyl-L-homoserine
-
 ?
N-butanoyl-L-homoserine lactone + H2O
-
 Enterobacter sp. CS66 N-butanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Enterobacter sp. CS66 N-decanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Enterobacter sp. CS66 N-hexanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O
-
 Enterobacter sp. CS66 N-octanoyl-L-homoserine
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Enterobacter sp. CS66

Synonyms

Synonyms Comment Organism
aiiE
-
 Enterobacter sp. CS66
quorum quenching AHL-lactonase
-
 Enterobacter sp. CS66

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
61.14
-
 N-butanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
69.43
-
 N-hexanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
80.07
-
 N-octanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
98.31
-
 N-decanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
101.5
-
 N-3-oxododecanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.61
-
 N-decanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
6.67
-
 N-3-oxododecanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
7.74
-
 N-octanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
8.81
-
 N-hexanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66
10.17
-
 N-butanoyl-L-homoserine lactone pH 7.2, 30°C Enterobacter sp. CS66