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Literature summary for 3.1.1.81 extracted from

  • Mascarenhas, R.; Thomas, P.W.; Wu, C.X.; Nocek, B.P.; Hoang, Q.Q.; Liu, D.; Fast, W.
    Structural and biochemical characterization of AidC, a quorum-quenching lactonase with atypical selectivity (2015), Biochemistry, 54, 4342-4353 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Chryseobacterium sp. StRB126

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray crystal structures ofAidC alone and with the product N-hexanoyl-L-homoserine are determined at resolutions of 1.09 and 1.67 A, respectively Chryseobacterium sp. StRB126

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 N-dodecanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
0.012
-
 N-decanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
0.047
-
 N-heptanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
0.061
-
 N-hexanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
0.083
-
 N-octanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
0.13
-
 N-3-oxooctanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
0.13
-
 N-pentanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
0.47
-
 N-butyryl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ AidC preparation contains 1.2 equiv of zinc ions per AidC monomer Chryseobacterium sp. StRB126

Organism

Organism UniProt Comment Textmining
Chryseobacterium sp. StRB126 I7HB71
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chryseobacterium sp. StRB126

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-3-oxooctanoyl-L-homoserine lactone + H2O
-
 Chryseobacterium sp. StRB126 N-3-oxooctanoyl-L-homoserine
-
 ?
N-butyryl-L-homoserine lactone + H2O
-
 Chryseobacterium sp. StRB126 N-butyryl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Chryseobacterium sp. StRB126 N-decanoyl-L-homoserine
-
 ?
N-dodecanoyl-L-homoserine lactone + H2O
-
 Chryseobacterium sp. StRB126 N-dodecanoyl-L-homoserine
-
 ?
N-heptanoyl-L-homoserine lactone + H2O
-
 Chryseobacterium sp. StRB126 N-heptanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Chryseobacterium sp. StRB126 N-hexanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O
-
 Chryseobacterium sp. StRB126 N-octanoyl-L-homoserine
-
 ?
N-pentanoyl-L-homoserine lactone + H2O
-
 Chryseobacterium sp. StRB126 N-pentanoyl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
AidC
-
 Chryseobacterium sp. StRB126
quorum-quenching lactonase
-
 Chryseobacterium sp. StRB126

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 N-dodecanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
2 8 N-3-oxooctanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
5.2
-
 N-decanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
39
-
 N-butyryl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
45
-
 N-octanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
57
-
 N-pentanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
59
-
 N-hexanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
80
-
 N-heptanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
57
-
 N-dodecanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
83
-
 N-butyryl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
220
-
 N-3-oxooctanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
430
-
 N-decanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
440
-
 N-pentanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
540
-
 N-octanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
970
-
 N-hexanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126
1700
-
 N-heptanoyl-L-homoserine lactone pH 7.5, temperature not specified in the publication Chryseobacterium sp. StRB126