Literature summary for 3.1.1.81 extracted from

Mayer, C.; Romero, M.; Muras, A.; Otero, A.
Aii20J, a wide-spectrum thermostable N-acylhomoserine lactonase from the marine bacterium Tenacibaculum sp. 20J, can quench AHL-mediated acid resistance in Escherichia coli (2015), Appl. Microbiol. Biotechnol., 99, 9523-9539 .

Search for more literature for 3.1.1.81

Application

Application	Comment	Organism
biotechnology	extreme temperature and pH resistance, resistance to proteases, wide substratum spectrum, and high specific activity enable the application of this QQ enzyme in a wide range of biotechnological applications	Tenacibaculum sp. 20J
drug development	potential use of Aii20J in the control of the expression of virulence and adaptation genes in important human pathogens	Tenacibaculum sp. 20J

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21(DE3) plysS	Tenacibaculum sp. 20J

General Stability

General Stability	Organism
the enzyme is resistant to alpha-chymotrypsin	Tenacibaculum sp. 20J
the enzyme is resistant to protease K	Tenacibaculum sp. 20J

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	SDS-PAGE	Tenacibaculum sp. 20J

Organism

Organism	UniProt	Comment	Textmining
Tenacibaculum sp. 20J	A0A0H3YS61	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Tenacibaculum sp. 20J

Subunits

Subunits	Comment	Organism
?	x * 35000, SDS-PAGE	Tenacibaculum sp. 20J

Synonyms

Synonyms	Comment	Organism
Aii20J	-	Tenacibaculum sp. 20J

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
100	-	10 min, the enzyme maintains its activity	Tenacibaculum sp. 20J

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Release 2023.1 (January 2023)