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Literature summary for 3.1.1.81 extracted from
- Design of N-acyl homoserine lactonase with high substrate specificity by a rational approach (2015), Appl. Microbiol. Biotechnol., 99, 4735-4742 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A206F | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 3.34% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| A206I | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 0.85% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| A206L | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 0.89% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| A206V | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 0.17% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| A206W | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 2.16% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| F64A | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 0.56% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| F64I | no activity with N-butyryl-L-homoserine lactone and N-decanoyl-L-homoserine lactone | Bacillus thuringiensis serovar kurstaki |
| F64L | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 1.98% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| F64V | no activity with N-butyryl-L-homoserine lactone and N-decanoyl-L-homoserine lactone | Bacillus thuringiensis serovar kurstaki |
| F64W | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 30.71% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| F64W/A206F | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 23.21% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| F64W/V69W | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 67.42% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| F64W/V69W/A206F | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 211.82% compared to 2.09% for wild-type enzyme. The mutant enzyme shows a 100fold shifted preference for N-butyryl-L-homoserine lactone | Bacillus thuringiensis serovar kurstaki |
| V69A | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 3.1% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| V69F | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 7.69% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| V69I | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 4.25% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| V69L | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 7.72% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| V69W | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 20.34% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
| V69W/A206F | the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 24.23% compared to 2.09% for wild-type enzyme | Bacillus thuringiensis serovar kurstaki |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0147 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206V | Bacillus thuringiensis serovar kurstaki |  |
| 0.0614 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206L | Bacillus thuringiensis serovar kurstaki | |
| 0.094 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69L | Bacillus thuringiensis serovar kurstaki | |
| 0.111 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/V69W | Bacillus thuringiensis serovar kurstaki | |
| 0.127 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 0.14 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206W | Bacillus thuringiensis serovar kurstaki | |
| 0.169 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206F | Bacillus thuringiensis serovar kurstaki | |
| 0.195 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69W | Bacillus thuringiensis serovar kurstaki | |
| 0.206 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69F | Bacillus thuringiensis serovar kurstaki | |
| 0.208 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64A | Bacillus thuringiensis serovar kurstaki | |
| 0.218 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69I | Bacillus thuringiensis serovar kurstaki | |
| 0.224 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 0.23 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W | Bacillus thuringiensis serovar kurstaki | |
| 0.315 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206I | Bacillus thuringiensis serovar kurstaki | |
| 0.358 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69A | Bacillus thuringiensis serovar kurstaki | |
| 0.385 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64L | Bacillus thuringiensis serovar kurstaki | |
| 0.419 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/V69W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 0.424 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, wild-type enzyme | Bacillus thuringiensis serovar kurstaki | |
| 1.3 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/V69W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 2.8 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 4.9 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/V69W | Bacillus thuringiensis serovar kurstaki | |
| 5.42 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69F | Bacillus thuringiensis serovar kurstaki | |
| 6.1 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 6.42 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69W | Bacillus thuringiensis serovar kurstaki | |
| 7.1 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64A | Bacillus thuringiensis serovar kurstaki | |
| 8.7 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206W | Bacillus thuringiensis serovar kurstaki | |
| 8.88 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206V | Bacillus thuringiensis serovar kurstaki | |
| 11.7 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206L | Bacillus thuringiensis serovar kurstaki | |
| 12.6 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206F | Bacillus thuringiensis serovar kurstaki | |
| 13 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69I | Bacillus thuringiensis serovar kurstaki | |
| 13.4 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69L | Bacillus thuringiensis serovar kurstaki | |
| 14.3 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69A | Bacillus thuringiensis serovar kurstaki | |
| 16.1 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W | Bacillus thuringiensis serovar kurstaki | |
| 27.3 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206I | Bacillus thuringiensis serovar kurstaki | |
| 27.5 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, wild-type enzyme | Bacillus thuringiensis serovar kurstaki | |
| 39.5 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64L | Bacillus thuringiensis serovar kurstaki | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thuringiensis serovar kurstaki | P0CJ63 | - |
- |
| Bacillus thuringiensis serovar kurstaki HD-263 | P0CJ63 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-butyryl-L-homoserine lactone + H2O | the kcat/Km of wild-type enzyme for N-decanoyl-L-homoserine lactone is about 50fold higher than that for N-butyryl-L-homoserine lactone. The triple mutant (F64W/V69W/A206F) shows a 100fold shifted preference for N-butyryl-L-homoserine lactone | Bacillus thuringiensis serovar kurstaki | N-butyryl-L-homoserine | - |
? | |
| N-butyryl-L-homoserine lactone + H2O | the kcat/Km of wild-type enzyme for N-decanoyl-L-homoserine lactone is about 50fold higher than that for N-butyryl-L-homoserine lactone. The triple mutant (F64W/V69W/A206F) shows a 100fold shifted preference for N-butyryl-L-homoserine lactone | Bacillus thuringiensis serovar kurstaki HD-263 | N-butyryl-L-homoserine | - |
? | |
| N-decanoyl-L-homoserine lactone + H2O | the kcat/Km of wild-type enzyme for N-decanoyl-L-homoserine lactone is about 50fold higher than that for N-butyryl-L-homoserine lactone. The triple mutant (F64W/V69W/A206F) shows a 100fold shifted preference for N-butyryl-L-homoserine lactone | Bacillus thuringiensis serovar kurstaki | N-decanoyl-L-homoserine | - |
? | |
| N-decanoyl-L-homoserine lactone + H2O | the kcat/Km of wild-type enzyme for N-decanoyl-L-homoserine lactone is about 50fold higher than that for N-butyryl-L-homoserine lactone. The triple mutant (F64W/V69W/A206F) shows a 100fold shifted preference for N-butyryl-L-homoserine lactone | Bacillus thuringiensis serovar kurstaki HD-263 | N-decanoyl-L-homoserine | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.57 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64A | Bacillus thuringiensis serovar kurstaki | |
| 1.05 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206V | Bacillus thuringiensis serovar kurstaki | |
| 1.07 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206V | Bacillus thuringiensis serovar kurstaki | |
| 2.98 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64A | Bacillus thuringiensis serovar kurstaki | |
| 3.06 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206I | Bacillus thuringiensis serovar kurstaki | |
| 4.16 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206I | Bacillus thuringiensis serovar kurstaki | |
| 7.41 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W | Bacillus thuringiensis serovar kurstaki | |
| 7.83 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/V69W | Bacillus thuringiensis serovar kurstaki | |
| 8.63 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206L | Bacillus thuringiensis serovar kurstaki | |
| 8.72 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/V69W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 12.5 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69L | Bacillus thuringiensis serovar kurstaki | |
| 14.6 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206L | Bacillus thuringiensis serovar kurstaki | |
| 16.4 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 19.9 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69F | Bacillus thuringiensis serovar kurstaki | |
| 21.1 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 25.9 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69W | Bacillus thuringiensis serovar kurstaki | |
| 27.3 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69I | Bacillus thuringiensis serovar kurstaki | |
| 29 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64L | Bacillus thuringiensis serovar kurstaki | |
| 38.5 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206W | Bacillus thuringiensis serovar kurstaki | |
| 40.2 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69F | Bacillus thuringiensis serovar kurstaki | |
| 42 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206F | Bacillus thuringiensis serovar kurstaki | |
| 51.7 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206W | Bacillus thuringiensis serovar kurstaki | |
| 59.1 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64L | Bacillus thuringiensis serovar kurstaki | |
| 59.4 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/V69W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 61.5 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 69.3 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69I | Bacillus thuringiensis serovar kurstaki | |
| 75 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69A | Bacillus thuringiensis serovar kurstaki | |
| 89 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, wild-type enzyme | Bacillus thuringiensis serovar kurstaki | |
| 93.2 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69A | Bacillus thuringiensis serovar kurstaki | |
| 104 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206F | Bacillus thuringiensis serovar kurstaki | |
| 121 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, wild-type enzyme | Bacillus thuringiensis serovar kurstaki | |
| 138 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69L | Bacillus thuringiensis serovar kurstaki | |
| 159 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W | Bacillus thuringiensis serovar kurstaki | |
| 174 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69W | Bacillus thuringiensis serovar kurstaki | |
| 192 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 236 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/V69W | Bacillus thuringiensis serovar kurstaki | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0809 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64A | Bacillus thuringiensis serovar kurstaki | |
| 0.112 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206I | Bacillus thuringiensis serovar kurstaki | |
| 0.121 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206V | Bacillus thuringiensis serovar kurstaki | |
| 1.25 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206L | Bacillus thuringiensis serovar kurstaki | |
| 1.5 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64L | Bacillus thuringiensis serovar kurstaki | |
| 4.38 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, wild-type enzyme | Bacillus thuringiensis serovar kurstaki | |
| 5.33 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69I | Bacillus thuringiensis serovar kurstaki | |
| 5.94 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206W | Bacillus thuringiensis serovar kurstaki | |
| 6.51 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69A | Bacillus thuringiensis serovar kurstaki | |
| 7.43 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69F | Bacillus thuringiensis serovar kurstaki | |
| 8.3 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206F | Bacillus thuringiensis serovar kurstaki | |
| 9.88 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W | Bacillus thuringiensis serovar kurstaki | |
| 10.4 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69L | Bacillus thuringiensis serovar kurstaki | |
| 13.2 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206I | Bacillus thuringiensis serovar kurstaki | |
| 14.3 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64A | Bacillus thuringiensis serovar kurstaki | |
| 20.8 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/V69W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 21.9 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 27 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69W | Bacillus thuringiensis serovar kurstaki | |
| 31.4 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 32.1 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W | Bacillus thuringiensis serovar kurstaki | |
| 44.1 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/V69W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 47.7 | - |
N-butyryl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/V69W | Bacillus thuringiensis serovar kurstaki | |
| 70.8 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/V69W | Bacillus thuringiensis serovar kurstaki | |
| 71.4 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206V | Bacillus thuringiensis serovar kurstaki | |
| 75.2 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64L | Bacillus thuringiensis serovar kurstaki | |
| 94.2 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme F64W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 96.6 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69F | Bacillus thuringiensis serovar kurstaki | |
| 125 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69I | Bacillus thuringiensis serovar kurstaki | |
| 130 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69W/A206F | Bacillus thuringiensis serovar kurstaki | |
| 133 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69W | Bacillus thuringiensis serovar kurstaki | |
| 134 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69L | Bacillus thuringiensis serovar kurstaki | |
| 141 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206L | Bacillus thuringiensis serovar kurstaki | |
| 210 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, wild-type enzyme | Bacillus thuringiensis serovar kurstaki | |
| 210 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme V69A | Bacillus thuringiensis serovar kurstaki | |
| 248 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206F | Bacillus thuringiensis serovar kurstaki | |
| 275 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication, mutant enzyme A206W | Bacillus thuringiensis serovar kurstaki | |
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Release 2023.1 (January 2023)
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