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Literature summary for 3.1.1.81 extracted from
- The quorum-quenching lactonase from Alicyclobacter acidoterrestris purification, kinetic characterization, crystallization and crystallographic analysis (2017), Acta Crystallogr. Sect. F, 73, 476-480 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Alicyclobacillus acidoterrestris |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting-drop vapor diffusion, X-ray diffraction data collected at 1.65 A resolution | Alicyclobacillus acidoterrestris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0105 | - |
N-butanoyl-L-homoserine lactone | pH 8.3, 25°C | Alicyclobacillus acidoterrestris |  |
| 0.014 | - |
N-3-oxododecanoyl-L-homoserine lactone | pH 8.3, 25°C | Alicyclobacillus acidoterrestris | |
| 0.0496 | - |
N-decanoyl-L-homoserine lactone | pH 8.3, 25°C | Alicyclobacillus acidoterrestris | |
| 0.0827 | - |
N-hexanoyl-L-homoserine lactone | pH 8.3, 25°C | Alicyclobacillus acidoterrestris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32500 | - |
SDS-PAGE | Alicyclobacillus acidoterrestris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alicyclobacillus acidoterrestris | T0BMH6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Alicyclobacillus acidoterrestris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Alicyclobacillus acidoterrestris | N-3-oxododecanoyl-L-homoserine | - |
? | |
| N-butanoyl-L-homoserine lactone + H2O | - |
Alicyclobacillus acidoterrestris | N-butanoyl-L-homoserine | - |
? | |
| N-decanoyl-L-homoserine lactone + H2O | - |
Alicyclobacillus acidoterrestris | N-decanoyl-L-homoserine | - |
? | |
| N-hexanoyl-L-homoserine lactone + H2O | - |
Alicyclobacillus acidoterrestris | N-hexanoyl-L-homoserine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32500, SDS-PAGE | Alicyclobacillus acidoterrestris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AaL | - |
Alicyclobacillus acidoterrestris |
| quorum-quenching lactonase | - |
Alicyclobacillus acidoterrestris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
Tm-value | Alicyclobacillus acidoterrestris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.03 | - |
N-3-oxododecanoyl-L-homoserine lactone | pH 8.3, 25°C | Alicyclobacillus acidoterrestris | |
| 5.13 | - |
N-decanoyl-L-homoserine lactone | pH 8.3, 25°C | Alicyclobacillus acidoterrestris | |
| 13.54 | - |
N-butanoyl-L-homoserine lactone | pH 8.3, 25°C | Alicyclobacillus acidoterrestris | |
| 13.97 | - |
N-hexanoyl-L-homoserine lactone | pH 8.3, 25°C | Alicyclobacillus acidoterrestris | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.103 | - |
N-decanoyl-L-homoserine lactone | pH 8.3, 25°C | Alicyclobacillus acidoterrestris | |
| 0.169 | - |
N-hexanoyl-L-homoserine lactone | pH 8.3, 25°C | Alicyclobacillus acidoterrestris | |
| 0.36 | - |
N-3-oxododecanoyl-L-homoserine lactone | pH 8.3, 25°C | Alicyclobacillus acidoterrestris | |
| 1.29 | - |
N-butanoyl-L-homoserine lactone | pH 8.3, 25°C | Alicyclobacillus acidoterrestris | |
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