Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Rhodotorula mucilaginosa |
Crystallization (Comment) | Organism |
---|---|
crystal structure of AidH at 1.29 A resolution and the structures of AidH-C4-HS (N-butanoyl-L-homoserine), mutant S102G AidH-C6-HSL (N-hexanoyl-L-homoserine lactone) and mutant E219G AidH-C6-HS (N-hexanoyl-L-homoserine) complexes at resolutions of 1.09, 1.33 and 1.11 A, respectively, are determined | Rhodotorula mucilaginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula mucilaginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Rhodotorula mucilaginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(3-hydroxyhexanoyl)-L-homoserine lactone + H2O | - |
Rhodotorula mucilaginosa | N-(3-hydroxyhexanoyl)-L-homoserine | - |
? | |
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O | - |
Rhodotorula mucilaginosa | N-(3-oxo-N-hexanoyl)-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | - |
Rhodotorula mucilaginosa | N-hexanoyl-L-homoserine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AHL-lactonase | - |
Rhodotorula mucilaginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Rhodotorula mucilaginosa |