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Literature summary for 3.1.1.81 extracted from

  • Vinoj, G.; Vaseeharan, B.; Thomas, S.; Spiers, A.; Shanthi, S.
    Quorum-quenching activity of the AHL-lactonase from Bacillus licheniformis DAHB1 inhibits Vibrio biofilm formation in vitro and reduces shrimp intestinal colonisation and mortality (2014), Mar. Biotechnol., 16, 707-715.
    View publication on PubMed
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 calculated from cDNA Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Bacillus licheniformis

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-(3-oxo-N-hexanoyl)-L-homoserine
-
 ?
N-(3-oxo-octanoyl)-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-(3-oxo-octanoyl)-L-homoserine
-
 ?
N-butanoyl-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-butanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-decanoyl-L-homoserine
-
 ?
N-dodecanoyl-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-dodecanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-hexanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-octanoyl-L-homoserine
-
 ?
N-tetradecanoyl-L-homoserine lactone + H2O
-
 Bacillus licheniformis N-tetradecanoyl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
AHL-lactonase
-
 Bacillus licheniformis
AiiA
-
 Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Bacillus licheniformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50
-
 Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 retains 90% activity after incubation at 60-80 °C for 60min Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bacillus licheniformis

pH Range

pH Minimum pH Maximum Comment Organism
7 8
-
 Bacillus licheniformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 12 retains more than 80% of the initial activity after incubation at pH 6.0-12.0 for 60 min Bacillus licheniformis

General Information

General Information Comment Organism
physiological function purified recombinant AiiA inhibits vibrio biofilm development in a cover slip assay and significantly attenuates infection and mortality in shrimps reared in a recirculation aquaculture system Bacillus licheniformis