Literature summary for 3.1.1.81 extracted from

Liu, C.F.; Liu, D.; Momb, J.; Thomas, P.W.; Lajoie, A.; Petsko, G.A.; Fast, W.; Ringe, D.
A phenylalanine clamp controls substrate specificity in the quorum-quenching metallo-gamma-lactonase from Bacillus thuringiensis (2013), Biochemistry, 52, 1603-1610.

Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Bacillus thuringiensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of AiiA F107W mutant determined in the presence and absence of N-decanoyl- L-homoserine is reported	Bacillus thuringiensis

Protein Variants

Protein Variants	Comment	Organism
F107W	crystal structure of AiiA F107W mutant determined in the presence and absence of N-decanoyl- L-homoserine is reported. Mutant shows lower catalytic activity for all substrates tested. Catalytic efficacies (kcat/Km) decrease by 2 to 3 orders of magnitude with N-pentanoyl-L-homoserine lactone displaying the highest decrease	Bacillus thuringiensis
F64C/F68C	in the absence of DTT, the enzymatic activity of F64C/F68C AiiA is impaired as compared to that of the wild-type enzyme, and this reduction is more severe for the long N-octanoyl-L-homoserine lactone substrate than for the short N-pentanoyl-L-homoserine lactone substrate. The kcat value is decreased by 14 and 41fold and the KM by 2 and 4fold, resulting in 21 and 158fold decreases in kcat/KM for the N-pentanoyl-L-homoserine lactone and N-octanoyl-L-homoserine lactone substrates, respectively. The addition of DTT not only partially restores enzyme activity but also recovers the characteristic substrate specificity of the wild-type enzyme	Bacillus thuringiensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.14	-	N-decanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
0.15	-	N-decanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
0.18	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
0.26	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F64C/F68C, presence of DTT	Bacillus thuringiensis
0.3	-	N-heptanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
0.7	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F64C/F68C, absence of DTT	Bacillus thuringiensis
0.7	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type, presence of DTT	Bacillus thuringiensis
0.8	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
1.6	-	N-heptanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
1.8	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F64C/F68C, presence of DTT	Bacillus thuringiensis
2.1	-	N-hexanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
3	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
4.5	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F64C/F68C, absence of DTT	Bacillus thuringiensis
6	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
6	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type, presence of DTT	Bacillus thuringiensis
10	-	N-hexanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis

Organism

Organism	UniProt	Comment	Textmining
Bacillus thuringiensis	A3FJ64	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-decanoyl-L-homoserine lactone + H2O	-	Bacillus thuringiensis	N-decanoyl-L-homoserine	-	?
N-heptanoyl-L-homoserine lactone + H2O	-	Bacillus thuringiensis	N-heptanoyl-L-homoserine	-	?
N-hexanoyl-L-homoserine lactone + H2O	-	Bacillus thuringiensis	N-hexanoyl-L-homoserine	-	?
N-octanoyl-L-homoserine lactone + H2O	-	Bacillus thuringiensis	N-octanoyl-L-homoserine	-	?
N-pentanoyl-L-homoserine lactone + H2O	-	Bacillus thuringiensis	N-pentanoyl-L-homoserine	-	?

Synonyms

Synonyms	Comment	Organism
AiiA	-	Bacillus thuringiensis
autoinducer inactivator A	-	Bacillus thuringiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.08	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
0.257	-	N-hexanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
0.3	-	N-heptanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
0.32	-	N-decanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
0.46	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
0.83	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F64C/F68C, absence of DTT	Bacillus thuringiensis
3	6	N-hexanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
3	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F64C/F68C, absence of DTT	Bacillus thuringiensis
6.1	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F64C/F68C, presence of DTT	Bacillus thuringiensis
8.2	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F64C/F68C, presence of DTT	Bacillus thuringiensis
34	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
34	-	N-heptanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
43	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
45	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type, presence of DTT	Bacillus thuringiensis
54	-	N-decanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
57	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type, presence of DTT	Bacillus thuringiensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Bacillus thuringiensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.012	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
0.026	-	N-hexanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
0.19	-	N-heptanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
0.58	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
0.67	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F64C/F68C, absence of DTT	Bacillus thuringiensis
1.2	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F64C/F68C, absence of DTT	Bacillus thuringiensis
2.1	-	N-decanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F107W	Bacillus thuringiensis
3.4	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F64C/F68C, presence of DTT	Bacillus thuringiensis
7.5	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type, presence of DTT	Bacillus thuringiensis
14	-	N-pentanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
17	-	N-hexanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
32	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, mutant F64C/F68C, presence of DTT	Bacillus thuringiensis
81	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type, presence of DTT	Bacillus thuringiensis
110	-	N-heptanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
190	-	N-octanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis
390	-	N-decanoyl-L-homoserine lactone	pH 7.5, temperature not specified in the publication, wild-type	Bacillus thuringiensis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)