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Literature summary for 3.1.1.81 extracted from

  • Ng, F.S.; Wright, D.M.; Seah, S.Y.
    Characterization of a phosphotriesterase-like lactonase from Sulfolobus solfataricus and its immobilization for disruption of quorum sensing (2010), Appl. Environ. Microbiol., 77, 1181-1186.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Pseudomonas putida KT2440 Saccharolobus solfataricus

General Stability

General Stability Organism
when the enzyme is immobilized on nanoalumina membranes 25% of the activity is retained upon immobilization Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
0.16
-
 N-(3-oxooctanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
0.17
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
5.6
-
 N-(3-oxohexanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 2.2 Co2+ per active site in the enzyme that is purified from recombinant Pseudomonas putida Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97VT7
-
-
Saccharolobus solfataricus P2 Q97VT7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(3-oxodecanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus N-(3-oxodecanoyl)-L-homoserine
-
 ?
N-(3-oxodecanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus P2 N-(3-oxodecanoyl)-L-homoserine
-
 ?
N-(3-oxododecanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus N-(3-oxododecanoyl)-L-homoserine
-
 ?
N-(3-oxododecanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus P2 N-(3-oxododecanoyl)-L-homoserine
-
 ?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus N-(3-oxohexanoyl)-L-homoserine
-
 ?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus P2 N-(3-oxohexanoyl)-L-homoserine
-
 ?
N-(3-oxooctanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus N-(3-oxooctanoyl)-L-homoserine
-
 ?
N-(3-oxooctanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus P2 N-(3-oxooctanoyl)-L-homoserine
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
N-acyl homoserine lactonase
-
 Saccharolobus solfataricus
SSO2522 locus name Saccharolobus solfataricus
SsoPox bifunctional enzyme with organophosphate hydrolase and N-acyl homoserine lactonase activities Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52
-
 N-(3-oxohexanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
0.95
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
1
-
 N-(3-oxooctanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
1.5
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.093
-
 N-(3-oxohexanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
5.5
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
6.6
-
 N-(3-oxooctanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
290
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus