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Literature summary for 3.1.1.81 extracted from
- High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture (2010), Microb. Cell Fact., 9, 39.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 112.2% activity at 1 mM | Bacillus thuringiensis serovar kyushuensis |  |
| EDTA | 103.6% activity at 10 mM | Bacillus thuringiensis serovar kyushuensis | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain GS115 | Bacillus thuringiensis serovar kyushuensis |
General Stability
| General Stability | Organism |
|---|---|
| after incubation with trypsin, subtilisin A, collagenase and proleather at 37°C for 30 or 60 min, the enzyme maintains or enhances its enzymatic activity | Bacillus thuringiensis serovar kyushuensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 1 mM | Bacillus thuringiensis serovar kyushuensis | |
| Cr3+ | 29.1% residual activity at 1 mM | Bacillus thuringiensis serovar kyushuensis | |
| Cu2+ | 68.5% residual activity at 1 mM | Bacillus thuringiensis serovar kyushuensis | |
| Hg2+ | complete inhibition at 1 mM | Bacillus thuringiensis serovar kyushuensis | |
| Li+ | 65.8% residual activity at 10 mM | Bacillus thuringiensis serovar kyushuensis | |
| Pb2+ | 87.5% residual activity at 10 mM | Bacillus thuringiensis serovar kyushuensis | |
| SDS | complete inhibition at 10 mM | Bacillus thuringiensis serovar kyushuensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 117.5% activity at 10 mM | Bacillus thuringiensis serovar kyushuensis | |
| Fe3+ | 104.8% activity at 1 mM | Bacillus thuringiensis serovar kyushuensis | |
| K+ | 109.3% activity at 10 mM | Bacillus thuringiensis serovar kyushuensis | |
| Li+ | 115.4% activity at 1 mM | Bacillus thuringiensis serovar kyushuensis | |
| Mg2+ | 108.4% activity at 10 mM | Bacillus thuringiensis serovar kyushuensis | |
| Mn2+ | 108.4% activity at 10 mM | Bacillus thuringiensis serovar kyushuensis | |
| Na+ | 109.5% activity at 1 mM | Bacillus thuringiensis serovar kyushuensis | |
| Ni2+ | 103.4% activity at 1 mM | Bacillus thuringiensis serovar kyushuensis | |
| Pb2+ | 108.6% activity at 1 mM | Bacillus thuringiensis serovar kyushuensis | |
| Zn2+ | 109.5% activity at 10 mM | Bacillus thuringiensis serovar kyushuensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28140 | - |
x * 28140, calculated from amino acid sequence | Bacillus thuringiensis serovar kyushuensis |
| 31500 | - |
x * 31500, deglycosylated enzyme, based on SDS-PAGE | Bacillus thuringiensis serovar kyushuensis |
| 33600 | - |
x * 33600, glycosylated enzyme, based on SDS-PAGE | Bacillus thuringiensis serovar kyushuensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thuringiensis serovar kyushuensis | D8VDY4 | - |
- |
| Bacillus thuringiensis serovar kyushuensis B546 | D8VDY4 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Bacillus thuringiensis serovar kyushuensis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and anion exchange chromatography | Bacillus thuringiensis serovar kyushuensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.995 | - |
purified enzyme, using N-(3-oxooctanoyl)-L-homoserine lactone as the substrate, at pH 8.0 and 25°C | Bacillus thuringiensis serovar kyushuensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, purified enzyme, 3 months, 1.6% loss of activity | Bacillus thuringiensis serovar kyushuensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-(3-oxohexanoyl)-L-homoserine lactone + H2O | - |
Bacillus thuringiensis serovar kyushuensis | L-homoserine lactone + 3-oxohexanoate | - |
? | |
| N-(3-oxohexanoyl)-L-homoserine lactone + H2O | - |
Bacillus thuringiensis serovar kyushuensis B546 | L-homoserine lactone + 3-oxohexanoate | - |
? | |
| N-(3-oxooctanoyl)-L-homoserine lactone + H2O | - |
Bacillus thuringiensis serovar kyushuensis | L-homoserine lactone + 3-oxooctanoate | - |
? | |
| N-decanoyl-L-homoserine lactone + H2O | best substrate | Bacillus thuringiensis serovar kyushuensis | L-homoserine lactone + decanoate | - |
? | |
| N-decanoyl-L-homoserine lactone + H2O | best substrate | Bacillus thuringiensis serovar kyushuensis B546 | L-homoserine lactone + decanoate | - |
? | |
| N-dodecanoyl-L-homoserine lactone + H2O | - |
Bacillus thuringiensis serovar kyushuensis | L-homoserine lactone + dodecanoate | - |
? | |
| N-dodecanoyl-L-homoserine lactone + H2O | - |
Bacillus thuringiensis serovar kyushuensis B546 | L-homoserine lactone + dodecanoate | - |
? | |
| N-hexanoyl-L-homoserine lactone + H2O | - |
Bacillus thuringiensis serovar kyushuensis | L-homoserine lactone + hexanoate | - |
? | |
| N-hexanoyl-L-homoserine lactone + H2O | - |
Bacillus thuringiensis serovar kyushuensis B546 | L-homoserine lactone + hexanoate | - |
? | |
| N-octanoyl-L-homoserine lactone + H2O | worst substrate | Bacillus thuringiensis serovar kyushuensis | L-homoserine lactone + octanoate | - |
? | |
| N-octanoyl-L-homoserine lactone + H2O | worst substrate | Bacillus thuringiensis serovar kyushuensis B546 | L-homoserine lactone + octanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28140, calculated from amino acid sequence | Bacillus thuringiensis serovar kyushuensis |
| ? | x * 31500, deglycosylated enzyme, based on SDS-PAGE | Bacillus thuringiensis serovar kyushuensis |
| ? | x * 33600, glycosylated enzyme, based on SDS-PAGE | Bacillus thuringiensis serovar kyushuensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AHL-lactonase | - |
Bacillus thuringiensis serovar kyushuensis |
| AiiA | - |
Bacillus thuringiensis serovar kyushuensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Bacillus thuringiensis serovar kyushuensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
35 | at 0°C and 20-35°C, the enzyme maintains more than 60% of the highest activity at 20°C | Bacillus thuringiensis serovar kyushuensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
recombinant enzyme retains more than 80% of the initial activity after preincubation at 70°C for 30 min | Bacillus thuringiensis serovar kyushuensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus thuringiensis serovar kyushuensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.9 | more than 73% of the maximum activity at pH 6.5-8.9 | Bacillus thuringiensis serovar kyushuensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 12 | the enzyme is stable at pH 6.0-12.0, retaining more than 70% activity after pre-incubation at 37°C for 1 h | Bacillus thuringiensis serovar kyushuensis |
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