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Literature summary for 3.1.1.81 extracted from

  • Thomas, P.W.; Fast, W.
    Heterologous overexpression, purification, and in vitro characterization of AHL lactonases (2011), Methods Mol. Biol., 692, 275-290.
    View publication on PubMed
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus thuringiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 N-hexanoyl-L-homoserine lactone Mn2+-form of AHL lactonase, at pH 7.4 and at 28°C Bacillus thuringiensis
0.24
-
 N-hexanoyl-L-homoserine lactone Cd2+-form of AHL lactonase, at pH 7.4 and at 28°C Bacillus thuringiensis
0.36
-
 N-hexanoyl-L-homoserine lactone Co2+-form of AHL lactonase, at pH 7.4 and at 28°C Bacillus thuringiensis
5.6
-
 N-hexanoyl-L-homoserine lactone Zn2+-form of AHL lactonase, at pH 7.4 and at 28°C Bacillus thuringiensis

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose-agarose resin column chromatography and DEAE-Sepharose column chromatography Bacillus thuringiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
-
 Bacillus thuringiensis N-(3-oxooctanoyl)-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Bacillus thuringiensis N-hexanoyl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
AHL lactonase
-
 Bacillus thuringiensis
AiiA
-
 Bacillus thuringiensis
N-acyl-L-homoserine lactone lactonase
-
 Bacillus thuringiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
91
-
 N-hexanoyl-L-homoserine lactone Zn2+-form of AHL lactonase, at pH 7.4 and at 28°C Bacillus thuringiensis
330
-
 N-hexanoyl-L-homoserine lactone Mn2+-form of AHL lactonase, at pH 7.4 and at 28°C Bacillus thuringiensis
480
-
 N-hexanoyl-L-homoserine lactone Cd2+-form of AHL lactonase, at pH 7.4 and at 28°C Bacillus thuringiensis
510
-
 N-hexanoyl-L-homoserine lactone Co2+-form of AHL lactonase, at pH 7.4 and at 28°C Bacillus thuringiensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16
-
 N-hexanoyl-L-homoserine lactone Zn2+-form of AHL lactonase, at pH 7.4 and at 28°C Bacillus thuringiensis
1400
-
 N-hexanoyl-L-homoserine lactone Co2+-form of AHL lactonase, at pH 7.4 and at 28°C Bacillus thuringiensis
1800
-
 N-hexanoyl-L-homoserine lactone Mn2+-form of AHL lactonase, at pH 7.4 and at 28°C Bacillus thuringiensis
2000
-
 N-hexanoyl-L-homoserine lactone Cd2+-form of AHL lactonase, at pH 7.4 and at 28°C Bacillus thuringiensis