Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Parageobacillus caldoxylosilyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Parageobacillus caldoxylosilyticus | the AHL-degrading enzyme catalyzes the lactone ring opening of N-3-oxohexanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone by hydrolyzing the lactones | ? | - |
? | |
additional information | Parageobacillus caldoxylosilyticus YS-8 | the AHL-degrading enzyme catalyzes the lactone ring opening of N-3-oxohexanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone by hydrolyzing the lactones | ? | - |
? | |
N-3-oxo-dodecanoyl-L-homoserine lactone + H2O | Parageobacillus caldoxylosilyticus | - |
N-3-oxo-dodecanoyl-L-homoserine | - |
? | |
N-3-oxo-hexanoyl-L-homoserine lactone + H2O | Parageobacillus caldoxylosilyticus | lower activity | N-3-oxo-hexanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | Parageobacillus caldoxylosilyticus | - |
N-decanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | Parageobacillus caldoxylosilyticus YS-8 | - |
N-decanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | Parageobacillus caldoxylosilyticus | lower activity | N-hexanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | Parageobacillus caldoxylosilyticus YS-8 | lower activity | N-hexanoyl-L-homoserine | - |
? | |
N-octanoyl-L-homoserine lactone + H2O | Parageobacillus caldoxylosilyticus | lower activity | N-octanoyl-L-homoserine | - |
? | |
N-octanoyl-L-homoserine lactone + H2O | Parageobacillus caldoxylosilyticus YS-8 | lower activity | N-octanoyl-L-homoserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Parageobacillus caldoxylosilyticus | - |
- |
- |
Parageobacillus caldoxylosilyticus YS-8 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 201.5fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, gel filtration, and another step of anion exchange chromatography | Parageobacillus caldoxylosilyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
256 | - |
purified enzyme, substrate N-3-oxo-dodecanoyl-L-homoserine lactone, pH 6.5, 50°C | Parageobacillus caldoxylosilyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, overview | Parageobacillus caldoxylosilyticus | ? | - |
? | |
additional information | the AHL-degrading enzyme catalyzes the lactone ring opening of N-3-oxohexanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone by hydrolyzing the lactones | Parageobacillus caldoxylosilyticus | ? | - |
? | |
additional information | substrate specificity, overview | Parageobacillus caldoxylosilyticus YS-8 | ? | - |
? | |
additional information | the AHL-degrading enzyme catalyzes the lactone ring opening of N-3-oxohexanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone by hydrolyzing the lactones | Parageobacillus caldoxylosilyticus YS-8 | ? | - |
? | |
N-3-oxo-dodecanoyl-L-homoserine lactone + H2O | - |
Parageobacillus caldoxylosilyticus | N-3-oxo-dodecanoyl-L-homoserine | - |
? | |
N-3-oxo-hexanoyl-L-homoserine lactone + H2O | lower activity | Parageobacillus caldoxylosilyticus | N-3-oxo-hexanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Parageobacillus caldoxylosilyticus | N-decanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | - |
Parageobacillus caldoxylosilyticus YS-8 | N-decanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | lower activity | Parageobacillus caldoxylosilyticus | N-hexanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | lower activity | Parageobacillus caldoxylosilyticus YS-8 | N-hexanoyl-L-homoserine | - |
? | |
N-octanoyl-L-homoserine lactone + H2O | lower activity | Parageobacillus caldoxylosilyticus | N-octanoyl-L-homoserine | - |
? | |
N-octanoyl-L-homoserine lactone + H2O | lower activity | Parageobacillus caldoxylosilyticus YS-8 | N-octanoyl-L-homoserine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Parageobacillus caldoxylosilyticus |
Synonyms | Comment | Organism |
---|---|---|
AHL lactonase | - |
Parageobacillus caldoxylosilyticus |
AHL-degrading enzyme | - |
Parageobacillus caldoxylosilyticus |
N-acylhomoserine lactonase | - |
Parageobacillus caldoxylosilyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Parageobacillus caldoxylosilyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | activity range, 30% of maximal activity at 80°C, profile, overview | Parageobacillus caldoxylosilyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified enzyme, 3 h, 100% of maximal activity remaining | Parageobacillus caldoxylosilyticus |
50 | - |
purified enzyme, 3 h, 80% of maximal activity remaining | Parageobacillus caldoxylosilyticus |
60 | - |
purified enzyme, 3 h, 50% of maximal activity remaining | Parageobacillus caldoxylosilyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Parageobacillus caldoxylosilyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | activity range, profile, overview | Parageobacillus caldoxylosilyticus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in quorum sensing, a regulatory mechanism of cell-to-cell communication in response to environmental conditions | Parageobacillus caldoxylosilyticus |