Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.81 extracted from

  • Wang, W.Z.; Morohoshi, T.; Ikenoya, M.; Someya, N.; Ikeda, T.
    AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum (2010), Appl. Environ. Microbiol., 76, 2524-2530.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
gene aiiM, cloning from the genomic library, DNA and amino acid sequence determination and analysis, expression as a maltose binding protein-fusion protein in Escherichia coli strain DH5alpha Microbacterium testaceum

Protein Variants

Protein Variants Comment Organism
additional information expression of Microbacterium testaceum AiiM in the plant pathogen Pectobacterium carotovorum subsp. carotovorum reduces the pectinase activity markedly and attenuated soft rot symptoms on Solanum tuberosum tuber slices Microbacterium testaceum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 x * 74000, recombinant MBP-fusion enzyme, SDS-PAGE Microbacterium testaceum

Organism

Organism UniProt Comment Textmining
Microbacterium testaceum C6L862 isolated from the leaf surface of Solanum tuberosum, gene aiiM
-
Microbacterium testaceum StLB037 C6L862 isolated from the leaf surface of Solanum tuberosum, gene aiiM
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information strain StLB037 grows at temperatures ranging from 10°C to 60°C, reaching its optimum temperature at 37°C Microbacterium testaceum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homoserine lactone + H2O 20% of the activity with N-decanoyl-L-homoserine lactone Microbacterium testaceum L-homoserine
-
 ?
L-homoserine lactone + H2O 20% of the activity with N-decanoyl-L-homoserine lactone Microbacterium testaceum StLB037 L-homoserine
-
 ?
additional information poor activity with N-butyryl-L-homoserine lactone Microbacterium testaceum ?
-
 ?
additional information poor activity with N-butyryl-L-homoserine lactone Microbacterium testaceum StLB037 ?
-
 ?
N-decanoyl-L-homoserine lactone + H2O best substrate Microbacterium testaceum N-decanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O best substrate Microbacterium testaceum StLB037 N-decanoyl-L-homoserine
-
 ?
N-dodecanoyl-L-homoserine lactone + H2O
-
 Microbacterium testaceum N-dodecanoyl-L-homoserine
-
 ?
N-dodecanoyl-L-homoserine lactone + H2O
-
 Microbacterium testaceum StLB037 N-dodecanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O
-
 Microbacterium testaceum N-octanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O
-
 Microbacterium testaceum StLB037 N-octanoyl-L-homoserine
-
 ?

Subunits

Subunits Comment Organism
? x * 74000, recombinant MBP-fusion enzyme, SDS-PAGE Microbacterium testaceum

Synonyms

Synonyms Comment Organism
AHL lactonase
-
 Microbacterium testaceum
AiiM
-
 Microbacterium testaceum
More the enzyme belongs to the alpha/beta hydrolase fold family Microbacterium testaceum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Microbacterium testaceum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 80 over 80% of maximal activity within the range of 20-60°C Microbacterium testaceum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Microbacterium testaceum

pH Range

pH Minimum pH Maximum Comment Organism
4.2 8 no activity below pH 4.2, maximal activity at pH 8.0 Microbacterium testaceum

General Information

General Information Comment Organism
additional information the AHL-degrading enzyme catalyzes the lactone ring opening of N-acyl-L-homoserine lactones by hydrolyzing the lactones Microbacterium testaceum