Literature summary for 3.1.1.81 extracted from

Dong, Y.H.; Wang, L.H.; Xu, J.L.; Zhang, H.B.; Zhang, X.F.; Zhang, L.H.
Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase (2001), Nature, 411, 813-817.

Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment)	Organism
expression of GST-tagged AiiA containing a thrombin cleavage site in Escherichia coli strain DH5alpha, construction of transgenic Solanum tuberosum and Nicotiana tabacum plants expressing gene aiiA	Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of transgenic Solanum tuberosum and Nicotiana tabacum plants via Agrobacterium tumefaciens transfection with Bacillus sp. gene aiiA inserted into their genome, the transgenic plants express the recombinant aiiA gene and show increased AHL-lactonase activity compared to the wild-type plants, as well as enhanced resistance to plant pathogen Erwinia carotovora, the recombinant enzyme might be trapped in microsomes	Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-acyl-(S)-homoserine lactone + H2O	Bacillus sp. (in: Bacteria)	the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, the inhibition of which is a feasible approach for prevention of bacterial infection	N-acyl-(S)-homoserine	-	?
N-acyl-(S)-homoserine lactone + H2O	Bacillus sp. (in: Bacteria) 240B1	the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, the inhibition of which is a feasible approach for prevention of bacterial infection	N-acyl-(S)-homoserine	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	Q9L8R8	isolated from soil, gene aiiA	-
Bacillus sp. (in: Bacteria) 240B1	Q9L8R8	isolated from soil, gene aiiA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-tagged AiiA from Escherichia coli strain DH5alpha by glutathione affinity chromatography, the GST-atg is cleaved off by thrombin	Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the substrate N-acyl-L-homoserine lactones show negligible enzyme-independent delactonization	Bacillus sp. (in: Bacteria)	?	-	?
additional information	the substrate N-acyl-L-homoserine lactones show negligible enzyme-independent delactonization	Bacillus sp. (in: Bacteria) 240B1	?	-	?
N-3-oxododecanoyl-L-homoserine lactone + H2O	the substrate regulates production of virulence determinants of the human pathogen Pseudomonas aeruginosa	Bacillus sp. (in: Bacteria)	N-3-oxododecanoyl-L-homoserine	product identification by mass spectrometry	?
N-3-oxododecanoyl-L-homoserine lactone + H2O	the substrate regulates production of virulence determinants of the human pathogen Pseudomonas aeruginosa	Bacillus sp. (in: Bacteria) 240B1	N-3-oxododecanoyl-L-homoserine	product identification by mass spectrometry	?
N-3-oxohexanoyl-L-homoserine lactone + H2O	-	Bacillus sp. (in: Bacteria)	N-3-oxohexanoyl-L-homoserine	product identification by mass spectrometry	?
N-3-oxohexanoyl-L-homoserine lactone + H2O	-	Bacillus sp. (in: Bacteria) 240B1	N-3-oxohexanoyl-L-homoserine	product identification by mass spectrometry	?
N-3-oxooctanoyl-L-homoserine lactone + H2O	the substrate controls Ti plasmid conjugal transfer in Agrobacterium tumefaciens	Bacillus sp. (in: Bacteria)	N-3-oxooctanoyl-L-homoserine	product identification by mass spectrometry	?
N-3-oxooctanoyl-L-homoserine lactone + H2O	the substrate controls Ti plasmid conjugal transfer in Agrobacterium tumefaciens	Bacillus sp. (in: Bacteria) 240B1	N-3-oxooctanoyl-L-homoserine	product identification by mass spectrometry	?
N-acyl-(S)-homoserine lactone + H2O	the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, the inhibition of which is a feasible approach for prevention of bacterial infection	Bacillus sp. (in: Bacteria)	N-acyl-(S)-homoserine	-	?
N-acyl-(S)-homoserine lactone + H2O	the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, the inhibition of which is a feasible approach for prevention of bacterial infection	Bacillus sp. (in: Bacteria) 240B1	N-acyl-(S)-homoserine	-	?
N-butanoyl-L-homoserine lactone + H2O	the substrate regulates production of virulence determinants of the human pathogen Pseudomonas aeruginosa	Bacillus sp. (in: Bacteria)	N-butanoyl-L-homoserine	product identification by mass spectrometry	?

Synonyms

Synonyms	Comment	Organism
AHL-lactonase	-	Bacillus sp. (in: Bacteria)
delactonase	-	Bacillus sp. (in: Bacteria)
N-acyl homoserine lactonase	-	Bacillus sp. (in: Bacteria)
quorum-quenching enzyme	-	Bacillus sp. (in: Bacteria)

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)