Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is not affected by EDTA, 2,2'-bipyridine, and o-phenanthroline at 2 mM | Bacillus sp. (in: Bacteria) |
Cloned (Comment) | Organism |
---|---|
gene aiiA, expression of wild-type and mutant enzymes in Escherichia coli as GST-tagged proteins | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
D108S | site-directed mutagenesis, the mutant shows no activity, and altered secondary structure | Bacillus sp. (in: Bacteria) |
H106S | site-directed mutagenesis, the mutant shows 53.5% of wild-type enzyme activity, and altered secondary structure | Bacillus sp. (in: Bacteria) |
H109S | site-directed mutagenesis, the mutant shows no activity, and altered secondary structure | Bacillus sp. (in: Bacteria) |
H169S | site-directed mutagenesis, the mutant shows 53.1% of wild-type enzyme activity, and altered secondary structure | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 0.2 mM | Bacillus sp. (in: Bacteria) | |
Cr2+ | 72% inhibition at 2 mM | Bacillus sp. (in: Bacteria) | |
Cu2+ | complete inhibition at 0.2 mM | Bacillus sp. (in: Bacteria) | |
Fe2+ | 48% inhibition at 2 mM | Bacillus sp. (in: Bacteria) | |
additional information | enzyme is not affected by EDTA, 2,2'-bipyridine, and o-phenanthroline at 2 mM | Bacillus sp. (in: Bacteria) | |
Pb2+ | 67% inhibition at 2 mM | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics | Bacillus sp. (in: Bacteria) | |
0.0014 | - |
N-3-oxodecanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
0.0023 | - |
N-3-oxooctanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
0.0026 | - |
N-octanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
0.003 | - |
N-3-oxohexanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
0.0038 | - |
N-hexanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
0.0047 | - |
N-3-oxobutanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
0.0051 | - |
N-butanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
0.0075 | - |
N-3-hydroxybutanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by Mg2+, Ca2+, Mn2+, Co2+, Ni2+, Zn2+, and Cd2+, the enzyme contains the putative zinc-binding motif 104HXHXDH109, but is no metallohydrolase since it does not contain and require Zn2+ or other metal ions, only trace amounts of Zn2+, the 104HXHXDH109 sequence is a catalytic motif of the AHL-lactonase | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus sp. (in: Bacteria) | N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence | ? | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | Bacillus sp. (in: Bacteria) | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism | N-acyl-(S)-homoserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli by glutathione affinity chromatography | Bacillus sp. (in: Bacteria) |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine | residues H106, D108, and H109, as well as H169 are important for catalytic activity | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus sp. (in: Bacteria) |
Storage Stability | Organism |
---|---|
4°C or 21°C, purified recombinant enzyme, 10 days, 99% remaining activity | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | substrate specificity, poor activity with non-acyl lactones and no activity with non-cyclic esters, but strong activity with all N-acyl-(S)-homoserine lactones of different chain length and nature, the amide group and the ketone at C1 position of the substrate's acyl chain might be important for substrate-enzyme interaction, overview | Bacillus sp. (in: Bacteria) | ? | - |
? | |
N-3-hydroxybutanoyl-L-homoserine lactone + H2O | - |
Bacillus sp. (in: Bacteria) | N-3-hydroxybutanoyl-L-homoserine | - |
? | |
N-3-oxobutanoyl-L-homoserine lactone + H2O | - |
Bacillus sp. (in: Bacteria) | N-3-oxobutanoyl-L-homoserine | - |
? | |
N-3-oxodecanoyl-L-homoserine lactone + H2O | high activity | Bacillus sp. (in: Bacteria) | N-3-oxodecanoyl-L-homoserine | - |
? | |
N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Bacillus sp. (in: Bacteria) | N-3-oxododecanoyl-L-homoserine | - |
? | |
N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Bacillus sp. (in: Bacteria) | N-3-oxohexanoyl-L-homoserine | - |
? | |
N-3-oxooctanoyl-L-homoserine lactone + H2O | high activity | Bacillus sp. (in: Bacteria) | N-3-oxooctanoyl-L-homoserine | - |
? | |
N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism | Bacillus sp. (in: Bacteria) | N-acyl-(S)-homoserine | - |
? | |
N-butanoyl-L-homoserine lactone + H2O | high activity | Bacillus sp. (in: Bacteria) | N-butanoyl-L-homoserine | - |
? | |
N-decanoyl-L-homoserine lactone + H2O | high activity | Bacillus sp. (in: Bacteria) | N-decanoyl-L-homoserine | - |
? | |
N-hexanoyl-L-homoserine lactone + H2O | best substrate | Bacillus sp. (in: Bacteria) | N-hexanoyl-L-homoserine | - |
? | |
N-octanoyl-L-homoserine lactone + H2O | high activity | Bacillus sp. (in: Bacteria) | N-octanoyl-L-homoserine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | analysis of secondary wild-type and mutant enzyme structures, comparison, overview | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
AHL-lactonase | - |
Bacillus sp. (in: Bacteria) |
quorum-quenching enzyme | - |
Bacillus sp. (in: Bacteria) |
quorum-quenching N-acyl homoserine lactone lactonase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
6 | 37 | - |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
purified recombinant wild-type enzyme, completely stable below | Bacillus sp. (in: Bacteria) |
45 | - |
purified recombinant wild-type enzyme, inactivation, 2 h | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20.2 | - |
N-3-oxodecanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
22.2 | - |
N-3-oxooctanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
22.7 | - |
N-3-oxohexanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
27.5 | - |
N-octanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
28.6 | - |
N-3-oxobutanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
29.3 | - |
N-3-hydroxybutanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
35.7 | - |
N-hexanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) | |
37.6 | - |
N-butanoyl-L-homoserine lactone | pH 7.4, 22°C, recombinant wild-type enzyme | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
broad optimum of pH 7.0 to pH 9.0 | Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | complete loss of activity at pH 5.5 and above pH 9.0, pH-profile | Bacillus sp. (in: Bacteria) |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
4.7 |