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Literature summary for 3.1.1.81 extracted from

  • Wang, L.H.; Weng, L.X.; Dong, Y.H.; Zhang, L.H.
    Specificity and enzyme kinetics of the quorum-quenching N-acyl homoserine lactone lactonase (AHL-lactonase) (2004), J. Biol. Chem., 279, 13645-13651.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme is not affected by EDTA, 2,2'-bipyridine, and o-phenanthroline at 2 mM Bacillus sp. (in: Bacteria)

Cloned(Commentary)

Cloned (Comment) Organism
gene aiiA, expression of wild-type and mutant enzymes in Escherichia coli as GST-tagged proteins Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants Comment Organism
D108S site-directed mutagenesis, the mutant shows no activity, and altered secondary structure Bacillus sp. (in: Bacteria)
H106S site-directed mutagenesis, the mutant shows 53.5% of wild-type enzyme activity, and altered secondary structure Bacillus sp. (in: Bacteria)
H109S site-directed mutagenesis, the mutant shows no activity, and altered secondary structure Bacillus sp. (in: Bacteria)
H169S site-directed mutagenesis, the mutant shows 53.1% of wild-type enzyme activity, and altered secondary structure Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 0.2 mM Bacillus sp. (in: Bacteria)
Cr2+ 72% inhibition at 2 mM Bacillus sp. (in: Bacteria)
Cu2+ complete inhibition at 0.2 mM Bacillus sp. (in: Bacteria)
Fe2+ 48% inhibition at 2 mM Bacillus sp. (in: Bacteria)
additional information enzyme is not affected by EDTA, 2,2'-bipyridine, and o-phenanthroline at 2 mM Bacillus sp. (in: Bacteria)
Pb2+ 67% inhibition at 2 mM Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics and thermodynamics Bacillus sp. (in: Bacteria)
0.0014
-
N-3-oxodecanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
0.0023
-
N-3-oxooctanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
0.0026
-
N-octanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
0.003
-
N-3-oxohexanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
0.0038
-
N-hexanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
0.0047
-
N-3-oxobutanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
0.0051
-
N-butanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
0.0075
-
N-3-hydroxybutanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect by Mg2+, Ca2+, Mn2+, Co2+, Ni2+, Zn2+, and Cd2+, the enzyme contains the putative zinc-binding motif 104HXHXDH109, but is no metallohydrolase since it does not contain and require Zn2+ or other metal ions, only trace amounts of Zn2+, the 104HXHXDH109 sequence is a catalytic motif of the AHL-lactonase Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus sp. (in: Bacteria) N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence ?
-
?
N-acyl-(S)-homoserine lactone + H2O Bacillus sp. (in: Bacteria) the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism N-acyl-(S)-homoserine
-
?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli by glutathione affinity chromatography Bacillus sp. (in: Bacteria)

Reaction

Reaction Comment Organism Reaction ID
an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine residues H106, D108, and H109, as well as H169 are important for catalytic activity Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bacillus sp. (in: Bacteria)

Storage Stability

Storage Stability Organism
4°C or 21°C, purified recombinant enzyme, 10 days, 99% remaining activity Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence Bacillus sp. (in: Bacteria) ?
-
?
additional information substrate specificity, poor activity with non-acyl lactones and no activity with non-cyclic esters, but strong activity with all N-acyl-(S)-homoserine lactones of different chain length and nature, the amide group and the ketone at C1 position of the substrate's acyl chain might be important for substrate-enzyme interaction, overview Bacillus sp. (in: Bacteria) ?
-
?
N-3-hydroxybutanoyl-L-homoserine lactone + H2O
-
Bacillus sp. (in: Bacteria) N-3-hydroxybutanoyl-L-homoserine
-
?
N-3-oxobutanoyl-L-homoserine lactone + H2O
-
Bacillus sp. (in: Bacteria) N-3-oxobutanoyl-L-homoserine
-
?
N-3-oxodecanoyl-L-homoserine lactone + H2O high activity Bacillus sp. (in: Bacteria) N-3-oxodecanoyl-L-homoserine
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
Bacillus sp. (in: Bacteria) N-3-oxododecanoyl-L-homoserine
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
Bacillus sp. (in: Bacteria) N-3-oxohexanoyl-L-homoserine
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O high activity Bacillus sp. (in: Bacteria) N-3-oxooctanoyl-L-homoserine
-
?
N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism Bacillus sp. (in: Bacteria) N-acyl-(S)-homoserine
-
?
N-butanoyl-L-homoserine lactone + H2O high activity Bacillus sp. (in: Bacteria) N-butanoyl-L-homoserine
-
?
N-decanoyl-L-homoserine lactone + H2O high activity Bacillus sp. (in: Bacteria) N-decanoyl-L-homoserine
-
?
N-hexanoyl-L-homoserine lactone + H2O best substrate Bacillus sp. (in: Bacteria) N-hexanoyl-L-homoserine
-
?
N-octanoyl-L-homoserine lactone + H2O high activity Bacillus sp. (in: Bacteria) N-octanoyl-L-homoserine
-
?

Subunits

Subunits Comment Organism
More analysis of secondary wild-type and mutant enzyme structures, comparison, overview Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
AHL-lactonase
-
Bacillus sp. (in: Bacteria)
quorum-quenching enzyme
-
Bacillus sp. (in: Bacteria)
quorum-quenching N-acyl homoserine lactone lactonase
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6 37
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
purified recombinant wild-type enzyme, completely stable below Bacillus sp. (in: Bacteria)
45
-
purified recombinant wild-type enzyme, inactivation, 2 h Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20.2
-
N-3-oxodecanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
22.2
-
N-3-oxooctanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
22.7
-
N-3-oxohexanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
27.5
-
N-octanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
28.6
-
N-3-oxobutanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
29.3
-
N-3-hydroxybutanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
35.7
-
N-hexanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)
37.6
-
N-butanoyl-L-homoserine lactone pH 7.4, 22°C, recombinant wild-type enzyme Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
broad optimum of pH 7.0 to pH 9.0 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
6 9 complete loss of activity at pH 5.5 and above pH 9.0, pH-profile Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria)
-
-
4.7