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Literature summary for 3.1.1.80 extracted from

  • Ruppert, M.; Woll, J.; Giritch, A.; Genady, E.; Ma, X.; Stöckigt, J.
    Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system (2005), Planta, 222, 888-898.
    View publication on PubMed
Search for more literature for 3.1.1.80

Cloned(Commentary)

Cloned (Comment) Organism
the expression in Escherichia coli failed although a wide range of conditions were tested. With a virus-based plant expression system, it was possible to express the enzyme functionally in leaves of Nicotiana benthamiana Domin. An extraordinarily high enzyme activity is detected in the Nicotiana tissue, which exceeds that in Rauvolfia cell suspension cultures about 20fold Rauvolfia verticillata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 17-O-acetylajmaline
-
 Rauvolfia verticillata
0.7
-
 17-O-acetylnorajmaline
-
 Rauvolfia verticillata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Rauvolfia verticillata

Organism

Organism UniProt Comment Textmining
Rauvolfia serpentina Q3MKY2 L. Benth. ex Kurz
-
Rauvolfia verticillata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rauvolfia verticillata

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Rauvolfia verticillata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.484
-
-
 Rauvolfia verticillata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17-O-acetylajmaline + H2O
-
 Rauvolfia verticillata ajmaline + acetate
-
 ?
17-O-acetylnorajmaline + H2O 70.2% of the activity with 17-O-acetylajmaline Rauvolfia verticillata norajmaline + acetate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Rauvolfia verticillata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
53
-
-
 Rauvolfia verticillata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Rauvolfia verticillata

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.8 pH 6.5 and pH 9.8: about 50% of maximal activity Rauvolfia verticillata

pI Value

Organism Comment pI Value Maximum pI Value
Rauvolfia verticillata calculated from sequence
-
 7.65