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Literature summary for 3.1.1.8 extracted from
- Purification and characterization of butyrylcholine-hydrolyzing enzyme from Pseudomonas polycolor (1976), Biochim. Biophys. Acta, 429, 817-827.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | - |
Pseudomonas aeruginosa |  |
| atropine | competitive | Pseudomonas aeruginosa | |
| CuSO4 | - |
Pseudomonas aeruginosa | |
| diisopropylphosphofluoridate | irreversible inhibition | Pseudomonas aeruginosa | |
| HgCl2 | - |
Pseudomonas aeruginosa | |
| homatropine | competitive | Pseudomonas aeruginosa | |
| iodoacetic acid | - |
Pseudomonas aeruginosa | |
| additional information | enzyme is inhibited by quaternary ammonium salts. The effect is influenced by the size of the quaternary ammonium ion | Pseudomonas aeruginosa | |
| p-chloromercuribenzoate | - |
Pseudomonas aeruginosa | |
| physostigmine | i.e. eserine; irreversible inhibition | Pseudomonas aeruginosa | |
| procaine | competitive | Pseudomonas aeruginosa | |
| quinidine | competitive | Pseudomonas aeruginosa | |
| Scopolamine | competitive | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
Butyrylcholine | pH 7.5, 25°C | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
IFO 3918 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas aeruginosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.1 M potassium phosphate buffer, pH 7.4, 1 month, no loss of activity | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoylcholine + H2O | - |
Pseudomonas aeruginosa | benzoate + choline | - |
? | |
| butyrylcholine + H2O | best substrate | Pseudomonas aeruginosa | butanoate + choline | - |
? | |
| butyrylthiocholine + H2O | good substrate | Pseudomonas aeruginosa | butanoate + thiocholine | - |
? | |
| ethyl n-butyrate + H2O | poor substrate | Pseudomonas aeruginosa | ethanol + n-butanoate | - |
? | |
| isoamyl isovalerate + H2O | poor substrate | Pseudomonas aeruginosa | isopentanol + isopentanoate | - |
? | |
| isoamyl n-butyrate + H2O | - |
Pseudomonas aeruginosa | isopentanol + n-butanoate | - |
? | |
| isoamyl propionate + H2O | - |
Pseudomonas aeruginosa | isopentanol + propanoate | - |
? | |
| isobutyl isovalerate + H2o | poor substrate | Pseudomonas aeruginosa | isobutanol + isopentanoate | - |
? | |
| isobutyl n-butyrate + H2O | - |
Pseudomonas aeruginosa | isobutanol + n-butanoate | - |
? | |
| isobutyl propionate + H2O | poor substrate | Pseudomonas aeruginosa | isobutanol + propanoate | - |
? | |
| methyl n-butyrate + H2O | poor substrate | Pseudomonas aeruginosa | methanol + n-butanoate | - |
? | |
| methyl n-valerate + H2O | poor substrate | Pseudomonas aeruginosa | methanol + n-pentanoate | - |
? | |
| additional information | butyryl esters are hydrolyzed at a greater rate than propionyl, pentanoyl, valeryl- or acetyl esters | Pseudomonas aeruginosa | ? | - |
? | |
| n-amyl acetate + H2O | poor substrate | Pseudomonas aeruginosa | n-pentanol + acetate | - |
? | |
| n-amyl n-butyrate + H2O | good substrate | Pseudomonas aeruginosa | n-pentanol + n-butanoate | - |
? | |
| n-butyl acetate + H2O | poor substrate | Pseudomonas aeruginosa | n-butanol + acetate | - |
? | |
| n-butyl n-butyrate + H2O | poor substrate | Pseudomonas aeruginosa | n-butanol + n-butanoate | - |
? | |
| n-hexyl acetate + H2O | poor substrate | Pseudomonas aeruginosa | n-hexanol + acetate | - |
? | |
| propionylcholine + H2O | good substrate | Pseudomonas aeruginosa | propanoate + choline | - |
? | |
| propionylthiocholine + H2O | - |
Pseudomonas aeruginosa | propanoate + thiocholine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| butyrylcholine-hydrolyzing enzyme | - |
Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
0.1 M phosphate buffer, pH 5.5, 4% loss of activity | Pseudomonas aeruginosa |
| 80 | - |
above 80°C, 10 min, 0.1 M phosphate buffer, pH 5.5, complete inactivation | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | optimum reactivity for hydrolysis of butyrylcholine as substrate | Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
5 min, 75°C, 90% loss of activity | Pseudomonas aeruginosa |
| 5.5 | 7 | 5 min, 75°C, stable between | Pseudomonas aeruginosa |
| 8.5 | - |
5 min, 75°C, 60% loss of activity | Pseudomonas aeruginosa |
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