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Literature summary for 3.1.1.8 extracted from
- Characterization and in vitro sensitivity of cholinesterases of gilthead seabream (Sparus aurata) to organophosphate pesticides (2017), Fish Physiol. Biochem., 43, 455-464 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azinphosmethyl-oxon | - |
Sparus aurata |  |
| dichlorvos | - |
Sparus aurata | |
| additional information | in vitro sensitivity of cholinesterases from gilthead seabream (Sparus aurata) to organophosphate pesticides, overview. Muscle BChE is found to be several orders of magnitude (from 2 to 4) more sensitive than brain and muscle acetylcholinesterase (AChE, EC 3.1.1.7) inhibition by dichlorvos and omethoate. Enzyme BChE does not show excess substrate inhibition | Sparus aurata | |
| omethoate | - |
Sparus aurata | |
| tetraisopropylpyrophosphoramide | iso-OMPA, enzyme BChE is selectively and completely inhibited | Sparus aurata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Sparus aurata | |
| 0.16 | - |
butyrylthiocholine | pH 7.4, 25°C, enzyme from muscle | Sparus aurata | |
| 0.163 | - |
butyrylthiocholine | pH 7.4, 25°C, enzyme from yolk-sac of seabream larvae stage | Sparus aurata | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butyrylcholine + H2O | Sparus aurata | - |
choline + butyrate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sparus aurata | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | low enzyme level | Sparus aurata | - |
| additional information | acetylcholinesterase (AChE, EC 3.1.1.7) is the enzyme present in the brain, whereas in muscle, a typical formof AChE is present along with an atypical form of butyrylcholinesterase (BChE) | Sparus aurata | - |
| muscle | - |
Sparus aurata | - |
| plasma cell | low enzyme level | Sparus aurata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylthiocholine + H2O | enzyme BChE may hydrolyze in some extent the substrate acetylthiocholine, ASCh | Sparus aurata | thiocholine + acetate | - |
? | |
| butyrylcholine + H2O | - |
Sparus aurata | choline + butyrate | - |
? | |
| butyrylthiocholine + H2O | - |
Sparus aurata | thiocholine + butyrate | - |
? | |
| additional information | BChE hydrolyzes a wider range of esters but preferentially S-butyrylthiocholine iodide (BSCh) | Sparus aurata | ? | - |
? | |
| propionylthiocholine + H2O | - |
Sparus aurata | thiocholine + propionate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BChE | - |
Sparus aurata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Sparus aurata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Sparus aurata |
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