Literature summary for 3.1.1.8 extracted from

Mennillo, E.; Casu, V.; Tardelli, F.; De Marchi, L.; Freitas, R.; Pretti, C.
Suitability of cholinesterase of polychaete Diopatra neapolitana as biomarker of exposure to pesticides In vitro characterization (2017), Comp. Biochem. Physiol. C, 191, 152-159 .

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Inhibitors

Inhibitors	Comment	Organism	Structure
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide	BW284c51	Diopatra neapolitana
Aldicarb	-	Diopatra neapolitana
Carbaryl	-	Diopatra neapolitana
carbofuran	-	Diopatra neapolitana
eserine	-	Diopatra neapolitana
methomyl	-	Diopatra neapolitana
tetra(monoisopropyl)diphosphotetramide	isoOMPA	Diopatra neapolitana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	substrate affinity (butyryl-, and propionylthiocholine), Michaelis-Menten kinetic modeling	Diopatra neapolitana
0.1237	-	butyrylthiocholine	pH 8.0, 25°C	Diopatra neapolitana
0.6254	-	propionylthiocholine	pH 8.0, 25°C	Diopatra neapolitana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
150000	-	the enzyme activity of S9 from Diopatra neapolitana incubated with PTChI reveals a main reactive band of about 150 kDa and a minor faint band of about 52 kDa, native PAGE	Diopatra neapolitana

Organism

Organism	UniProt	Comment	Textmining
Diopatra neapolitana	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	enzyme activity in whole body and different body segments (apical, intermediate, posterior), the rate of hydrolysis for acetyl- and propionylthiocholine is higher in the posterior segment than the apical/intermediate segments and whole body	Diopatra neapolitana	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.6	-	substrate butyrylthiocholine, pH 8.0, 25°C	Diopatra neapolitana
64.1	-	substrate propionylthiocholine, pH 8.0, 25°C	Diopatra neapolitana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
butyrylthiocholine + H2O	butyrylthiocholine iodide, low activity	Diopatra neapolitana	thiocholine + butyrate	-	?
propionylthiocholine + H2O	propionylthiocholine iodide, preferred substrate	Diopatra neapolitana	thiocholine + propionate	-	?

Subunits

Subunits	Comment	Organism
homotrimer	3 * 52000	Diopatra neapolitana

Synonyms

Synonyms	Comment	Organism
BTChI-ChE	-	Diopatra neapolitana
PTChI-ChE	-	Diopatra neapolitana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	37	the activity at 25°C is slightly higher compared to 37°C	Diopatra neapolitana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Diopatra neapolitana

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00011	-	inhibition of propionylthiocholine esterase activity, pH 8.0, 25°C	Diopatra neapolitana	Aldicarb
0.0002	-	inhibition of propionylthiocholine esterase activity, pH 8.0, 25°C	Diopatra neapolitana	eserine
0.00023	-	inhibition of propionylthiocholine esterase activity, pH 8.0, 25°C	Diopatra neapolitana	methomyl
0.00027	-	inhibition of propionylthiocholine esterase activity, pH 8.0, 25°C	Diopatra neapolitana	carbofuran
0.00312	-	inhibition of propionylthiocholine esterase activity, pH 8.0, 25°C	Diopatra neapolitana	Carbaryl

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Release 2023.1 (January 2023)