Any feedback?
Literature summary for 3.1.1.8 extracted from
- Cholinesterases in Gambusia yucatana biochemical characterization and its relationship with sex and total length (2016), Bull. Environ. Contam. Toxicol., 97, 776-780 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,5-bis-(4-allyldimethyl-ammoniumphenyl)-pentan-3-one dibromide | BW284c51 | Gambusia yucatana |  |
| eserine | - |
Gambusia yucatana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Gambusia yucatana | |
| 0.55 | - |
butyrylthiocholine | enzyme from muscle, pH 7.4, temperature not specified in the publication | Gambusia yucatana | |
| 1.24 | - |
butyrylthiocholine | enzyme from head, pH 7.4, temperature not specified in the publication | Gambusia yucatana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butyrylcholine + H2O | Gambusia yucatana | - |
choline + butyrate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gambusia yucatana | - |
from Yucatan Peninsula, Mexico | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| head | - |
Gambusia yucatana | - |
| additional information | the ChE presented in head and muscle is able to hydrolyze ASChI and BSChI obtaining higher Vmax and Km with ASChI. This suggests that the ChE present in head and muscle have high affinity for BSChI (ASChI Km < BSChI Km) but is poorly hydrolyzed. With ASChI as substrate, a relatively high enzyme activity is obtained (ASChI Vmax > BSChI Vmax), but it has a smaller affinity for the enzyme with respect to BSChI | Gambusia yucatana | - |
| muscle | - |
Gambusia yucatana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00595 | - |
crude enzyme, pH 7.4, temperature not specified in the publication | Gambusia yucatana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butyrylcholine + H2O | - |
Gambusia yucatana | choline + butyrate | - |
? | |
| butyrylthiocholine + H2O | - |
Gambusia yucatana | thiocholine + butyrate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BChE | - |
Gambusia yucatana |
| butyrylcholinesterase | - |
Gambusia yucatana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Gambusia yucatana |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00007 | - |
head enzyme, pH 7.4, temperature not specified in the publication | Gambusia yucatana | eserine | |
| 0.00009 | - |
muscle enzyme, pH 7.4, temperature not specified in the publication | Gambusia yucatana | eserine | |
| 0.00014 | - |
muscle enzyme, pH 7.4, temperature not specified in the publication | Gambusia yucatana | 1,5-bis-(4-allyldimethyl-ammoniumphenyl)-pentan-3-one dibromide | |
| 0.00037 | - |
head enzyme, pH 7.4, temperature not specified in the publication | Gambusia yucatana | 1,5-bis-(4-allyldimethyl-ammoniumphenyl)-pentan-3-one dibromide | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
