Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 1%, 130% of initial activity | uncultured bacterium | |
Tween 20 | 1%, 200% of initial activity | uncultured bacterium | |
Tween 80 | 1%, 160% of initial activity | uncultured bacterium |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | uncultured bacterium |
Crystallization (Comment) | Organism |
---|---|
structures of wild type and mutated Est22 with its product 4-nitrophenol, to 1.4-2.43 A. Est22 exhibits an alpha/beta-hydrolase fold consisting of a catalytic domain and a substrate-recognizing cap domain. Residues Ser188, Asp287, and His317 comprise the catalytic triad in the catalytic domain. 4-Nitrophenol occupies the substrate binding pocket and forms hydrogen bonds with adjacent residues Gly108, Gly109, and Gly189 | uncultured bacterium |
Protein Variants | Comment | Organism |
---|---|---|
D287A | active site mutant, significant decrease in activity, mutant forms oligomers instead of dimers | uncultured bacterium |
H317A | active site mutant, significant decrease in activity, mutant forms oligomers instead of dimers | uncultured bacterium |
S188A | active site mutant, significant decrease in activity | uncultured bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 10 mM, 40% loss of activity | uncultured bacterium | |
Cu2+ | 10 mM, complete loss of activity | uncultured bacterium | |
Ni2+ | 10 mM, 50% loss of activity | uncultured bacterium | |
SDS | 1%, complete loss of activity | uncultured bacterium | |
Zn2+ | 10 mM, complete loss of activity | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4899 | - |
4-nitrophenyl acetate | pH 7.5, 40°C | uncultured bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79100 | - |
gel filtration, multi-angle light scattering | uncultured bacterium |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 15%, complete loss of activity | uncultured bacterium |
dimethylformamide | 15%, 95% loss of activity | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | H6BDX1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | uncultured bacterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2065 | - |
substrate 4-nitrophenyl acetate, pH 7.5, 40°C | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
uncultured bacterium | 4-nitrophenol + acetate | - |
? | |
additional information | Est22 hydrolyzes 4-nitrophenyl esters up to C8, while the enzymatic activity decreases significantly with increasing chain length | uncultured bacterium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 238900, calculated from sequence including His-tag | uncultured bacterium |
Synonyms | Comment | Organism |
---|---|---|
Est22 | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
uncultured bacterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
95% of maximum activity | uncultured bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3160 | - |
4-nitrophenyl acetate | pH 7.5, 40°C | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
uncultured bacterium |