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Literature summary for 3.1.1.79 extracted from
- Functional characterization of hormone sensitive-like lipase from Bacillus halodurans synthesis and recovery of pNP-laurate with high yields (2017), Extremophiles, 21, 871-889 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Halalkalibacterium halodurans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: EDTa or EGTA, dithiothreitol at 5 mM | Halalkalibacterium halodurans | |
| Pb2+ | 5 mM, 65% residual activity | Halalkalibacterium halodurans |  |
| PMSF | 5 mM, 17% residual activity | Halalkalibacterium halodurans | |
| SDS | 5 mM, 63% residual activity | Halalkalibacterium halodurans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.363 | - |
4-nitrophenyl palmitate | pH 9.0, 60°C | Halalkalibacterium halodurans | |
| 0.376 | - |
4-nitrophenyl palmitate | pH 9.0, 40°C | Halalkalibacterium halodurans | |
| 41.66 | - |
trioleoyl glycerol | pH 9.0, 60°C | Halalkalibacterium halodurans | |
| 45.45 | - |
tripalmitoyl glycerol | pH 9.0, 60°C | Halalkalibacterium halodurans | |
| 84.75 | - |
tricaproyl glycerol | pH 9.0, 60°C | Halalkalibacterium halodurans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
gel filtration | Halalkalibacterium halodurans |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| hexane | fatty acid preference during triacylglyceride hydrolysis alters with the organic solvents. In 50% n-hexane, C12 and C18:1 are the major fatty acids released, whereas C4 is the major fatty acid released when the hydrolysis is carried out in n-butanol | Halalkalibacterium halodurans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halalkalibacterium halodurans | Q9KAN9 | - |
- |
| Halalkalibacterium halodurans DSM 18197 | Q9KAN9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Halalkalibacterium halodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-myristoyl-2-oleoyl-3-palmitoyl glycerol + H2O | racemic substrate | Halalkalibacterium halodurans | myristate + oleate + palmitate + ? | - |
? | |
| 4-nitrophenyl butanoate + H2O | - |
Halalkalibacterium halodurans | 4-nitrophenol + butanoate | - |
? | |
| 4-nitrophenyl butanoate + H2O | - |
Halalkalibacterium halodurans DSM 18197 | 4-nitrophenol + butanoate | - |
? | |
| 4-nitrophenyl caprate + H2O | - |
Halalkalibacterium halodurans | 4-nitrophenol + caprate | - |
? | |
| 4-nitrophenyl caprylate + H2O | - |
Halalkalibacterium halodurans | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl caprylate + H2O | - |
Halalkalibacterium halodurans DSM 18197 | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl laurate + H2O | best substrate | Halalkalibacterium halodurans | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl laurate + H2O | best substrate | Halalkalibacterium halodurans DSM 18197 | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl myristate + H2O | - |
Halalkalibacterium halodurans | 4-nitrophenol + myristate | - |
? | |
| 4-nitrophenyl myristate + H2O | - |
Halalkalibacterium halodurans DSM 18197 | 4-nitrophenol + myristate | - |
? | |
| 4-nitrophenyl palmitate + H2O | 70% of the activity with 4-nitrophenyl laurate | Halalkalibacterium halodurans | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | 70% of the activity with 4-nitrophenyl laurate | Halalkalibacterium halodurans DSM 18197 | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl stearate + H2O | - |
Halalkalibacterium halodurans | 4-nitrophenol + stearate | - |
? | |
| coconut oil + H2O | - |
Halalkalibacterium halodurans | laurate + ? | - |
? | |
| cod liver oil + H2O | - |
Halalkalibacterium halodurans | oleate + ? | - |
? | |
| palm oil + H2O | - |
Halalkalibacterium halodurans | palmitate + ? | - |
? | |
| tricaproyl glycerol + H2O | - |
Halalkalibacterium halodurans | dicaproyl glycerol + caprate | - |
? | |
| trioleoyl glycerol + H2O | - |
Halalkalibacterium halodurans | dioleoyl glycerol + oleate | - |
? | |
| tripalmitoyl glycerol + H2O | - |
Halalkalibacterium halodurans | dipalmitoyl glycerol + palmitate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42300, calculated from sequence | Halalkalibacterium halodurans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Blip | - |
Halalkalibacterium halodurans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Halalkalibacterium halodurans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
96 h, 57% residual activity | Halalkalibacterium halodurans |
| 90 | - |
half-life 200 min | Halalkalibacterium halodurans |
| 100 | - |
half-life 35 min | Halalkalibacterium halodurans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2763 | - |
tricaproyl glycerol | pH 9.0, 60°C | Halalkalibacterium halodurans | |
| 3078 | - |
tripalmitoyl glycerol | pH 9.0, 60°C | Halalkalibacterium halodurans | |
| 5280 | - |
trioleoyl glycerol | pH 9.0, 60°C | Halalkalibacterium halodurans | |
| 10930 | - |
4-nitrophenyl palmitate | pH 9.0, 40°C | Halalkalibacterium halodurans | |
| 15260 | - |
4-nitrophenyl palmitate | pH 9.0, 60°C | Halalkalibacterium halodurans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Halalkalibacterium halodurans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | more than 80% of maximum activity | Halalkalibacterium halodurans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 67.7 | - |
tripalmitoyl glycerol | pH 9.0, 60°C | Halalkalibacterium halodurans | |
| 126.7 | - |
trioleoyl glycerol | pH 9.0, 60°C | Halalkalibacterium halodurans | |
| 326 | - |
tricaproyl glycerol | pH 9.0, 60°C | Halalkalibacterium halodurans | |
| 29066 | - |
4-nitrophenyl palmitate | pH 9.0, 40°C | Halalkalibacterium halodurans | |
| 42033 | - |
4-nitrophenyl palmitate | pH 9.0, 60°C | Halalkalibacterium halodurans | |
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