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Literature summary for 3.1.1.79 extracted from

  • Dukunde, A.; Schneider, D.; Lu, M.; Brady, S.; Daniel, R.
    A novel, versatile family IV carboxylesterase exhibits high stability and activity in a broad pH spectrum (2017), Biotechnol. Lett., 39, 577-587 .
    View publication on PubMed
Search for more literature for 3.1.1.79

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol at 1% uncultured organism
DMSO increase in enzyme activity (130%) is observed at 25% v/v DMSO although at a higher concentration (50%), DMSO decreases enzyme activity to 72% uncultured organism
DTT at 1% uncultured organism

Cloned(Commentary)

Cloned (Comment) Organism
gene est06, screening of a forest soil metagenomic library, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, esterase (Est06) belonging to the hormone- sensitive lipase family of lipolytic enzymes is subcloned and heterologously expressed in Escherichia coli strain BL21(DE3) uncultured organism

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 45% inhibition at 1 mM uncultured organism
DMSO increase in enzyme activity (130%) is observed at 25% v/v DMSO although at a higher concentration (50%), DMSO decreases enzyme activity to 72% uncultured organism
Fe2+ 35% inhibition at 1 mM uncultured organism
additional information no inhibition by EDTA, poor effect by Triton X-100 uncultured organism
PMSF strong inhibition uncultured organism
SDS complete inactivation uncultured organism
Tween 20
-
 uncultured organism
Tween 80
-
 uncultured organism

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl moderate stability with NaCl up to 2.5 M with the residual activity being over 75%, 1 h, 25°C uncultured organism

Organism

Organism UniProt Comment Textmining
uncultured organism G3CRD1 a forest soil metagenome-derived gene
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.76
-
 purified recombinant enzyme, pH 7.0, 30°C, substrate 4-nitrophenyl valerate uncultured organism

Storage Stability

Storage Stability Organism
10-40°C, purified recombinant enzyme Est06 retains all its activity at 10, 25 and 30°C over the entire period. After six days of incubation at 40°C, esterase activity remains above 70%, considerable loss in Est06 activity (less than 65% residual activity) is observed only after the 10th day of incubation uncultured organism

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O high activity uncultured organism 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O high activity uncultured organism 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl caprate + H2O low activity uncultured organism 4-nitrophenol + caprate
-
 ?
4-nitrophenyl caproate + H2O
-
 uncultured organism 4-nitrophenol + caproate
-
 ?
4-nitrophenyl caprylate + H2O low activity uncultured organism 4-nitrophenol + caprylate
-
 ?
4-nitrophenyl laurate + H2O low activity uncultured organism 4-nitrophenol + laurate
-
 ?
4-nitrophenyl myristate + H2O low activity uncultured organism 4-nitrophenol + myristate
-
 ?
4-nitrophenyl palmitate + H2O low activity uncultured organism 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl valerate + H2O best substrate uncultured organism 4-nitrophenol + valerate
-
 ?
additional information the purified enzyme Est06 exhibits high affinity for acyl esters with short-chain fatty acids, and shows optimum activity with p-nitrophenyl valerate uncultured organism ?
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, about, sequence calculation, x* 35000, recombinant enzyme, SDS-PAGE uncultured organism
More tertiary structure prediction confirmed the presence of two alpha-helices, alpha1 and alpha2, that constitute the N-terminal cap domain followed by the alpha/beta fold of the catalytic domain. The hydrolase fold comprises eight beta-strands that form parallel structures, with Ser144 located between beta5 and alpha5, Glu238 between beta7 and alpha7 and His268 between beta8 and alpha9. Two cysteine residues, Cys46 and Cys170, which may participate in disulphide bridge formation uncultured organism

Synonyms

Synonyms Comment Organism
Est06
-
 uncultured organism
family IV carboxylesterase
-
 uncultured organism

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 uncultured organism

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 purified recombinant enzyme Est06 exhibits high stability at moderate temperatures by retaining all of its catalytic activity below 30°C over 13 days uncultured organism

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 uncultured organism

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 over 50% of maximal activity within this range uncultured organism

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 purified recombinant enzyme Est06 displays high stability within this range, at 25°C for 4 h uncultured organism

pI Value

Organism Comment pI Value Maximum pI Value
uncultured organism sequence calculation
-
 5.99

General Information

General Information Comment Organism
evolution the enzyme is a metagenome-derived member of the hormone-sensitive lipase family of lipolytic enzymes uncultured organism
additional information Est06 has a HGGG motif (residues 74 to 77) and a catalytic triad composed of Ser144, Glu238, and His268 uncultured organism