Activating Compound | Comment | Organism | Structure |
---|---|---|---|
betaine | it enhances the enzyme activity 2fold at 50°C and 3 M, and 1.5fold at 45°C and 1-3 M, while it reduces the enzyme activity at 35-40°C, by 20% at 40°C and 3 M, about 5% at 1 M, overview | Psychrobacter sp. | |
additional information | proline does not activate and thermally stabilize the enzyme at 35-50°C and concentrations of 1-3 M, but inhibits the enzyme activity | Psychrobacter sp. | |
Trimethylamine N-oxide | it enhances the enzyme activity 2.5fold at 50°C and 3 M, and 5fold at 45°C and 1 M, while it reduces the enzyme activity at 35-40°C, by 60% at 40°C and 3 M, 10% at 1 M, overview | Psychrobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
betaine | it enhances the enzyme activity 2fold at 50°C and 3 M, and 1.5fold at 45°C and 1-3 M, while it reduces the enzyme activity at 35-40°C, by 20% at 40°C and 3 M,about % at 1 M, overview | Psychrobacter sp. | |
proline | inhibits the enzyme at 35-50°C and concentrations of 1-3 M | Psychrobacter sp. | |
Trimethylamine N-oxide | it enhances the enzyme activity 2.5fold at 50°C and 3 M, and 5fold at 45°C and 1 M, while it reduces the enzyme activity at 35-40°C, by 60% at 40°C and 3 M, 10% at 1 M, overview | Psychrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Psychrobacter sp. | - |
- |
- |
Psychrobacter sp. TA144 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl pentanoate + H2O | - |
Psychrobacter sp. | 4-nitrophenol + pentanoate | - |
? | |
4-nitrophenyl pentanoate + H2O | - |
Psychrobacter sp. TA144 | 4-nitrophenol + pentanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cold-active hormone-sensitive lipase | - |
Psychrobacter sp. |
HSL | - |
Psychrobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 35 | activity range, enzyme without additives | Psychrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
proline does not activate and thermally stabilize the enzyme at 35-50°C and concentrations of 1-3 M, but inhibits the enzyme activity | Psychrobacter sp. |
35 | 50 | purified recombinant enzyme, pH 8.0, the enzyme activity is enhanced by 1-3 M trimethylamine N-oxide and betaine up to 5fold, overview. Denaturation temperature of the enzyme is 35.5°C, in presence of 20% glycerol it increases to 43.5°C, in presence of 20% glycerol and 1 M betaine to 44.5°C, and in presence of 20% glycerol and 1 M trimethylamine N-oxide to 50.5°C | Psychrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Psychrobacter sp. |