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Literature summary for 3.1.1.79 extracted from

  • De Santi, C.; Durante, L.; Vecchio, P.D.; Tutino, M.L.; Parrilli, E.; de Pascale, D.
    Thermal stabilization of psychrophilic enzymes: a case study of the cold-active hormone-sensitive lipase from Psychrobacter sp. TA144 (2012), Biotechnol. Prog., 28, 946-952.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
betaine it enhances the enzyme activity 2fold at 50°C and 3 M, and 1.5fold at 45°C and 1-3 M, while it reduces the enzyme activity at 35-40°C, by 20% at 40°C and 3 M, about 5% at 1 M, overview Psychrobacter sp.
additional information proline does not activate and thermally stabilize the enzyme at 35-50°C and concentrations of 1-3 M, but inhibits the enzyme activity Psychrobacter sp.
Trimethylamine N-oxide it enhances the enzyme activity 2.5fold at 50°C and 3 M, and 5fold at 45°C and 1 M, while it reduces the enzyme activity at 35-40°C, by 60% at 40°C and 3 M, 10% at 1 M, overview Psychrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
betaine it enhances the enzyme activity 2fold at 50°C and 3 M, and 1.5fold at 45°C and 1-3 M, while it reduces the enzyme activity at 35-40°C, by 20% at 40°C and 3 M,about % at 1 M, overview Psychrobacter sp.
proline inhibits the enzyme at 35-50°C and concentrations of 1-3 M Psychrobacter sp.
Trimethylamine N-oxide it enhances the enzyme activity 2.5fold at 50°C and 3 M, and 5fold at 45°C and 1 M, while it reduces the enzyme activity at 35-40°C, by 60% at 40°C and 3 M, 10% at 1 M, overview Psychrobacter sp.

Organism

Organism UniProt Comment Textmining
Psychrobacter sp.
-
-
-
Psychrobacter sp. TA144
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl pentanoate + H2O
-
Psychrobacter sp. 4-nitrophenol + pentanoate
-
?
4-nitrophenyl pentanoate + H2O
-
Psychrobacter sp. TA144 4-nitrophenol + pentanoate
-
?

Synonyms

Synonyms Comment Organism
cold-active hormone-sensitive lipase
-
Psychrobacter sp.
HSL
-
Psychrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 35 activity range, enzyme without additives Psychrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
proline does not activate and thermally stabilize the enzyme at 35-50°C and concentrations of 1-3 M, but inhibits the enzyme activity Psychrobacter sp.
35 50 purified recombinant enzyme, pH 8.0, the enzyme activity is enhanced by 1-3 M trimethylamine N-oxide and betaine up to 5fold, overview. Denaturation temperature of the enzyme is 35.5°C, in presence of 20% glycerol it increases to 43.5°C, in presence of 20% glycerol and 1 M betaine to 44.5°C, and in presence of 20% glycerol and 1 M trimethylamine N-oxide to 50.5°C Psychrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Psychrobacter sp.