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Literature summary for 3.1.1.79 extracted from

  • Soror, S.H.; Rao, R.; Cullum, J.
    Mining the genome sequence for novel enzyme activity: characterisation of an unusual member of the hormone-sensitive lipase family of esterases from the genome of Streptomyces coelicolor A3 (2) (2009), Protein Eng. Des. Sel., 22, 333-339.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed as a His-tagged fusion protein in Escherichia coli Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
V158A 4fold increase in activity with 4-nitrophenyl caproate, in contrast to wild-type mutant also shows activity against tributyrin. V158A mutant shows an improved thermostabilty compared with wild-type. V158A mutant shows a greater affinity to p-nitrophenyl acetate. Mutant is more sensitive to Cu2+, but less sensitive to Hg2+ compared to wild-type Streptomyces coelicolor
V158A/W87F significantly increased activity with 4-nitrophenyl butyrate in the double mutant, double mutant shows similar affinity to p-nitrophenyl acetate Streptomyces coelicolor
W87F mutation shows no effect on substrate specificity. Mutant is less thermostable compared to wild-type. V158A mutant shows an improved thermostabilty compared with wild-type. W87F mutant shows a lower affinity to p-nitrophenyl acetate compared to wild-type. Mutant is more sensitive to Cu2+ and Hg2+ compared to wild-type Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
2-propanol 80-90% inhibition Streptomyces coelicolor
Cu2+ 10 mM, 50-60% inhibition Streptomyces coelicolor
ethanol 80-90% inhibition Streptomyces coelicolor
Hg2+ 10 mM, 50-60% inhibition Streptomyces coelicolor
methanol 80-90% inhibition Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
4-nitrophenyl acetate mutant V158A, Vmax: 0.008 mM/min Streptomyces coelicolor
0.28
-
p-nitrophenyl acetate mutant V158A, Vmax: 0.008 mM/min Streptomyces coelicolor
0.54
-
1-naphthyl acetate Vmax: 0.019 mM/min Streptomyces coelicolor
0.54
-
Alpha-naphtyl acetate Vmax: 0.019 mM/min Streptomyces coelicolor
1.4
-
4-nitrophenyl acetate double mutant V158A/W87F, Vmax: 0.032 mM/min Streptomyces coelicolor
1.4
-
p-nitrophenyl acetate double mutant V158A/W87F, Vmax: 0.032 mM/min Streptomyces coelicolor
1.71
-
4-nitrophenyl acetate Vmax: 0.0094 mM/min Streptomyces coelicolor
1.71
-
p-nitrophenyl acetate Vmax: 0.0094 mM/min Streptomyces coelicolor
5.43
-
2-naphthyl acetate Vmax: 0.0168 mM/min Streptomyces coelicolor
7.42
-
4-nitrophenyl acetate mutant W87F, Vmax: 0.0065 mM/min Streptomyces coelicolor
7.42
-
p-nitrophenyl acetate mutant W87F, Vmax: 0.0065 mM/min Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 10 M, 40% stimulation Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
calculated from cDNA Streptomyces coelicolor
38600
-
SDS-PAGE Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O
-
Streptomyces coelicolor 1-naphthol + acetate
-
?
2-naphthyl acetate + H2O
-
Streptomyces coelicolor 2-naphthol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Streptomyces coelicolor 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Streptomyces coelicolor 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Streptomyces coelicolor ?
-
?
4-nitrophenyl caproate + H2O
-
Streptomyces coelicolor 4-nitrophenol + caproate
-
?
4-nitrophenyl caproate + H2O
-
Streptomyces coelicolor ?
-
?
alpha-naphtyl acetate + H2O
-
Streptomyces coelicolor ?
-
?

Synonyms

Synonyms Comment Organism
HSL
-
Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Streptomyces coelicolor
55
-
activity increases with temperature reaching a plateau at 55°C Streptomyces coelicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
enzyme retains 70% of its activity after preincubation 1 h at 55°C Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Streptomyces coelicolor
7.5
-
optimal activity Streptomyces coelicolor

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8
-
Streptomyces coelicolor

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 10 stable for 24 h at 20°C Streptomyces coelicolor