Cloned (Comment) | Organism |
---|---|
cloned and expressed as a His-tagged fusion protein in Escherichia coli | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
V158A | 4fold increase in activity with 4-nitrophenyl caproate, in contrast to wild-type mutant also shows activity against tributyrin. V158A mutant shows an improved thermostabilty compared with wild-type. V158A mutant shows a greater affinity to p-nitrophenyl acetate. Mutant is more sensitive to Cu2+, but less sensitive to Hg2+ compared to wild-type | Streptomyces coelicolor |
V158A/W87F | significantly increased activity with 4-nitrophenyl butyrate in the double mutant, double mutant shows similar affinity to p-nitrophenyl acetate | Streptomyces coelicolor |
W87F | mutation shows no effect on substrate specificity. Mutant is less thermostable compared to wild-type. V158A mutant shows an improved thermostabilty compared with wild-type. W87F mutant shows a lower affinity to p-nitrophenyl acetate compared to wild-type. Mutant is more sensitive to Cu2+ and Hg2+ compared to wild-type | Streptomyces coelicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-propanol | 80-90% inhibition | Streptomyces coelicolor | |
Cu2+ | 10 mM, 50-60% inhibition | Streptomyces coelicolor | |
ethanol | 80-90% inhibition | Streptomyces coelicolor | |
Hg2+ | 10 mM, 50-60% inhibition | Streptomyces coelicolor | |
methanol | 80-90% inhibition | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
4-nitrophenyl acetate | mutant V158A, Vmax: 0.008 mM/min | Streptomyces coelicolor | |
0.28 | - |
p-nitrophenyl acetate | mutant V158A, Vmax: 0.008 mM/min | Streptomyces coelicolor | |
0.54 | - |
1-naphthyl acetate | Vmax: 0.019 mM/min | Streptomyces coelicolor | |
0.54 | - |
Alpha-naphtyl acetate | Vmax: 0.019 mM/min | Streptomyces coelicolor | |
1.4 | - |
4-nitrophenyl acetate | double mutant V158A/W87F, Vmax: 0.032 mM/min | Streptomyces coelicolor | |
1.4 | - |
p-nitrophenyl acetate | double mutant V158A/W87F, Vmax: 0.032 mM/min | Streptomyces coelicolor | |
1.71 | - |
4-nitrophenyl acetate | Vmax: 0.0094 mM/min | Streptomyces coelicolor | |
1.71 | - |
p-nitrophenyl acetate | Vmax: 0.0094 mM/min | Streptomyces coelicolor | |
5.43 | - |
2-naphthyl acetate | Vmax: 0.0168 mM/min | Streptomyces coelicolor | |
7.42 | - |
4-nitrophenyl acetate | mutant W87F, Vmax: 0.0065 mM/min | Streptomyces coelicolor | |
7.42 | - |
p-nitrophenyl acetate | mutant W87F, Vmax: 0.0065 mM/min | Streptomyces coelicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 10 M, 40% stimulation | Streptomyces coelicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
calculated from cDNA | Streptomyces coelicolor |
38600 | - |
SDS-PAGE | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | - |
Streptomyces coelicolor | 1-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Streptomyces coelicolor | 2-naphthol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Streptomyces coelicolor | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Streptomyces coelicolor | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Streptomyces coelicolor | ? | - |
? | |
4-nitrophenyl caproate + H2O | - |
Streptomyces coelicolor | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl caproate + H2O | - |
Streptomyces coelicolor | ? | - |
? | |
alpha-naphtyl acetate + H2O | - |
Streptomyces coelicolor | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HSL | - |
Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces coelicolor |
55 | - |
activity increases with temperature reaching a plateau at 55°C | Streptomyces coelicolor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
enzyme retains 70% of its activity after preincubation 1 h at 55°C | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces coelicolor |
7.5 | - |
optimal activity | Streptomyces coelicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Streptomyces coelicolor |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | stable for 24 h at 20°C | Streptomyces coelicolor |