Literature summary for 3.1.1.79 extracted from

Shen, W.J.; Patel, S.; Natu, V.; Kraemer, F.B.
Mutational analysis of structural features of rat hormone-sensitive lipase (1998), Biochemistry, 37, 8973-8979.

Search for more literature for 3.1.1.79

Cloned(Commentary)

Cloned (Comment)	Organism
expression in CHO cells	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
S423A	99% loss of cholesteryl oleate hydrolysis, more than 80% loss of trioleoylglycerol hydrolysis and more than 90% loss of dioleoylglycerol hydrolysis	Rattus norvegicus
S563A	98% loss of cholesteryl oleate hydrolysis, more than 80% loss of trioleoylglycerol hydrolysis and more than 90% loss of dioleoylglycerol hydrolysis	Rattus norvegicus
S563A/S565A	70% loss of cholesteryl oleate hydrolysis, 10% loss of trioleoylglycerol hydrolysis and 80% loss of dioleoylglycerol hydrolysis	Rattus norvegicus
S563D	approx. 20% increase in cholesteryl oleate hydrolysis compared to wild-type	Rattus norvegicus
S565A	70% loss of cholesteryl oleate hydrolysis, 35% loss of trioleoylglycerol hydrolysis and 30% loss of dioleoylglycerol hydrolysis	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0034	-	trioleoylglycerol	37°C, pH 7.0	Rattus norvegicus
0.0047	-	cholesteryl oleate	37°C, pH 7.0	Rattus norvegicus
0.03	-	dioleoylglycerol	37°C, pH 7.0	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Rattus norvegicus	5829	-

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	-	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cholesteryl oleate + H2O	approx. 55% of activity with dioleoylglcerol	Rattus norvegicus	cholesterol + oleate	-	?
dioleoylglycerol + H2O	-	Rattus norvegicus	monooleoylglycerol + oleate	-	?
trioleoylglycerol + H2O	approx. 16% of activity with dioleoylglcerol	Rattus norvegicus	dioleoylglycerol + oleate	-	?

Synonyms

Synonyms	Comment	Organism
HSL	-	Rattus norvegicus

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Release 2023.1 (January 2023)