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Literature summary for 3.1.1.77 extracted from

  • Munford, R.S.; Hall, C.L.
    Purification of acyloxyacyl hydrolase, a leukocyte enzyme that removes secondary acyl chains from bacterial lipopolysaccharides (1989), J. Biol. Chem., 264, 15613-15619.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dimethylsulfate induces enzyme activity when added to the medium Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol complete inhibition at 0.1 M Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00055
-
lipopolysaccharide
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
large subunit, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens
52000 60000 native PAGE Homo sapiens
48000
-
SDS-PAGE of large subunit after N-glycanase treatment Homo sapiens
14000 20000 small subunit, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-(acyloxy)acyl group of bacterial toxin Homo sapiens detoxification of LPS 3-hydroxyacyl group of bacterial toxin + a fatty acid
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein both subunits glycosylated Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HL-60 cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000005
-
cell lysate Homo sapiens
1.555
-
purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(acyloxy)acyl group of bacterial toxin + H2O
-
Homo sapiens 3-hydroxyacyl group of bacterial toxin + a fatty acid
-
?
3-(acyloxy)acyl group of bacterial toxin detoxification of LPS Homo sapiens 3-hydroxyacyl group of bacterial toxin + a fatty acid
-
?
lipopolysaccharide + H2O
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
dimer alpha, beta, 1 * 50000 + 1 * 14000-20000, disulfide linked Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8 5
-
Homo sapiens