Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-propanol | 2fold activation at 5% (v/v) | Streptomyces venezuelae | |
additional information | higher concentrations of detergents show a significant inhibitory effect | Streptomyces venezuelae | |
SDS | 3fold activation at 0.01% (w/v) | Streptomyces venezuelae | |
Triton X-100 | activates 1.7fold at 0.005% (w/v), inhibits by 72% at 0.03% (w/v) | Streptomyces venezuelae | |
Tween 80 | activates1.8fold at 0.005% (w/v), inhibits 90% at 0.01% (w/v) | Streptomyces venezuelae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetic anhydride | slight inhibition at 10 mM | Streptomyces venezuelae | |
carbodiimide | slight inhibition at 10 mM | Streptomyces venezuelae | |
DTT | 69% inhibition at 10 mM, 30% at 1 mM | Streptomyces venezuelae | |
EDTA | 73% inhibition at 10 mM | Streptomyces venezuelae | |
iodoacetamide | slight inhibition at 10 mM | Streptomyces venezuelae | |
additional information | no inhibition by N-ethylmaleimide at 10 mM | Streptomyces venezuelae | |
NaN3 | slight inhibition at 10 mM | Streptomyces venezuelae | |
PMSF | 40% inhibition at 10 mM | Streptomyces venezuelae | |
Triton X-100 | activates 1.7fold at 0.005% (w/v), inhibits by 72% at 0.03% (w/v) | Streptomyces venezuelae | |
Tween 80 | activates1.8fold at 0.005% (w/v), inhibits 90% at 0.01% (w/v) | Streptomyces venezuelae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.122 | - |
4-nitrophenyl octanoate | pH 9.0, 37°C | Streptomyces venezuelae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces venezuelae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, SDS-PAGE | Streptomyces venezuelae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces venezuelae | - |
isolated from a soil sample taken at the Campus of the University of the Basque Country, Bizkaia, Spain | - |
Streptomyces venezuelae SO1 | - |
isolated from a soil sample taken at the Campus of the University of the Basque Country, Bizkaia, Spain | - |
Purification (Comment) | Organism |
---|---|
extracellualr enzyme 4.2fold to homogeneity | Streptomyces venezuelae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.1 | - |
purified enzyme, pH 9.5, 30°C, substrate poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) | Streptomyces venezuelae |
Storage Stability | Organism |
---|---|
-20 to -80°C, purified enzyme, 85% activity remaining after 6 months | Streptomyces venezuelae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl decanoate + H2O | high activity | Streptomyces venezuelae | ? | - |
? | |
4-nitrophenyl decanoate + H2O | high activity | Streptomyces venezuelae SO1 | ? | - |
? | |
4-nitrophenyl dodecanoate + H2O | high activity | Streptomyces venezuelae | ? | - |
? | |
4-nitrophenyl dodecanoate + H2O | high activity | Streptomyces venezuelae SO1 | ? | - |
? | |
4-nitrophenyl hexadecanoate + H2O | lower activity | Streptomyces venezuelae | ? | - |
? | |
4-nitrophenyl hexadecanoate + H2O | lower activity | Streptomyces venezuelae SO1 | ? | - |
? | |
4-nitrophenyl octanoate + H2O | best artificial substrate, the enzyme can hydrolyze various substrates for esterases, such as tributyrin and 4-nitrophenyl-alkanoates | Streptomyces venezuelae | ? | - |
? | |
4-nitrophenyl valerate + H2O | - |
Streptomyces venezuelae | ? | - |
? | |
additional information | the enzyme catalyzes the hydrolysis of poly-3-hydroxyoctanoate to monomeric units and the ring-opening polymerization of beta-butyrolactone and lactides, while epsilon-caprolactone and pentadecalactone are hardly polymerized, the enzyme shows esterase activity with tributyrin as substrate. It is unable of hydrolysing olive oil, and shows poor activity with 4-nitrophenyl esteras of acetate, butyrate, and octadecanoate | Streptomyces venezuelae | ? | - |
? | |
additional information | the enzyme catalyzes the hydrolysis of poly-3-hydroxyoctanoate to monomeric units and the ring-opening polymerization of beta-butyrolactone and lactides, while epsilon-caprolactone and pentadecalactone are hardly polymerized, the enzyme shows esterase activity with tributyrin as substrate. It is unable of hydrolysing olive oil, and shows poor activity with 4-nitrophenyl esteras of acetate, butyrate, and octadecanoate | Streptomyces venezuelae SO1 | ? | - |
? | |
poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Streptomyces venezuelae | (R)-3-hydroxyoctanoate + ? | the monomer of 3-hydroxyoctanoate and the trimer are detected as the main hydrolysis products, representing 28 and 35% of the total degradation products, respectively. Additionally, dimers as well as the trimer are also detected as minor products, longer periods of incubation increase the concentration of the monomer, whereas that of trimers decreases markedly | ? | |
poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) + H2O | latex suspension | Streptomyces venezuelae SO1 | (R)-3-hydroxyoctanoate + ? | the monomer of 3-hydroxyoctanoate and the trimer are detected as the main hydrolysis products, representing 28 and 35% of the total degradation products, respectively. Additionally, dimers as well as the trimer are also detected as minor products, longer periods of incubation increase the concentration of the monomer, whereas that of trimers decreases markedly | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, SDS-PAGE | Streptomyces venezuelae |
Synonyms | Comment | Organism |
---|---|---|
MCL-PHA depolymerase | - |
Streptomyces venezuelae |
medium-chain-length poly(3-hydroxyalkanoate) depolymerase | - |
Streptomyces venezuelae |
medium-chain-length polyhydroxyalkanoate depolymerase | - |
Streptomyces venezuelae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Streptomyces venezuelae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal inactivation of the enzyme followed first-order kinetics in all cases, a value of 48.7 kcal/mol is calculated for the activation energy of enzyme thermal inactivation | Streptomyces venezuelae |
30 | - |
purified enzyme, retains full initial activity after 24 h | Streptomyces venezuelae |
40 | - |
purified enzyme, retains 80% of its initial activity after 8 h | Streptomyces venezuelae |
45 | - |
purified enzyme, retains 50% of its initial activity after 8 h | Streptomyces venezuelae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | maximum activity in the alkaline pH-range | Streptomyces venezuelae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 11 | over 60% of maximal activity within this range | Streptomyces venezuelae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces venezuelae | isoelectric focusing | - |
5.9 |