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Literature summary for 3.1.1.76 extracted from
- Purification and characterization of an extracellular medium-chain length polyhydroxyalkanoate depolymerase from Thermus thermophilus HB8 (2011), Polym. Degrad. Stab., 96, 670-678.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetone | - |
Thermus thermophilus |  |
| methanol | - |
Thermus thermophilus | |
| additional information | no effect by 2-mercaptoethanol | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | slight inhibition | Thermus thermophilus | |
| additional information | no effect by 2-mercaptoethanol on enzyme activity | Thermus thermophilus | |
| PMSF | - |
Thermus thermophilus | |
| Triton X-100 | inhibition at high concentration | Thermus thermophilus | |
| Tween 20 | inhibition at high concentration | Thermus thermophilus | |
| Tween 80 | inhibition at high concentration | Thermus thermophilus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Thermus thermophilus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, SDS-PAGE | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(3-hydroxydecanoate-co-3-hydroxhyoctanoate) + H2O | Thermus thermophilus | - |
? | - |
? | |
| poly(3-hydroxydecanoate-co-3-hydroxhyoctanoate) + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
? | - |
? | |
| poly(3-hydroxyoctanoic acid) + H2O | Thermus thermophilus | - |
? | - |
? | |
| poly(3-hydroxyoctanoic acid) + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
the enzyme is encoded in parts of gene TTHA1605 | - |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
the enzyme is encoded in parts of gene TTHA1605 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the enzyme is purified from cell medium 10.4fold by acetone precipitation, hydrophobic interaction chromatography using Octyl-Sepharose CL-4B, and gel filtration to homogeneity | Thermus thermophilus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | cell growth on sodium octanoate | Thermus thermophilus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl octanoate + H2O | - |
Thermus thermophilus | octanoate + 4-nitrophenol | - |
? | |
| 4-nitrophenyl octanoate + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | octanoate + 4-nitrophenol | - |
? | |
| additional information | substrate specificity, overview, the extracellular mcl-PHA depolymerase is also capable of hydrolyzing various short- and medium-chain length 4-nitrophenyl-alkanoates, no activity with poly(3-hydroxybutyrate) | Thermus thermophilus | ? | - |
? | |
| additional information | substrate specificity, overview, the extracellular mcl-PHA depolymerase is also capable of hydrolyzing various short- and medium-chain length 4-nitrophenyl-alkanoates, no activity with poly(3-hydroxybutyrate) | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
| poly(3-hydroxydecanoate-co-3-hydroxhyoctanoate) + H2O | - |
Thermus thermophilus | ? | - |
? | |
| poly(3-hydroxydecanoate-co-3-hydroxhyoctanoate) + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
| poly(3-hydroxyoctanoic acid) + H2O | - |
Thermus thermophilus | ? | - |
? | |
| poly(3-hydroxyoctanoic acid) + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCL-PHA depolymerase | - |
Thermus thermophilus |
| medium-chain length polyhydroxyalkanoate depolymerase | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
with substrate 4-nitrophenyl octanoate | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Thermus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | the purified enzyme is most active at pH range of pH 7.0-9.0, while the maximal activity is observed at pH 8.5. At pH 6.0 and pH 11 the enzyme shows 34% and 7.5% of maximal activity, respectively | Thermus thermophilus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the mcl-PHA depolymerase from Thermus thermophilus is a distinct enzyme, which is different from those of other mcl-PHAdegrading bacteria | Thermus thermophilus |
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