Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.76 extracted from

  • de Eugenio, L.I.; Garcia, P.; Luengo, J.M.; Sanz, J.M.; Roman, J.S.; Garcia, J.L.; Prieto, M.A.
    Biochemical evidence that phaZ gene encodes a specific intracellular medium chain length polyhydroxyalkanoate depolymerase in Pseudomonas putida KT2442: characterization of a paradigmatic enzyme (2007), J. Biol. Chem., 282, 4951-4962.
    View publication on PubMed
Search for more literature for 3.1.1.76

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol
-
 Pseudomonas putida
mercaptoethanol
-
 Pseudomonas putida
phenylmethylsulfonyl fluoride
-
 Pseudomonas putida
SDS
-
 Pseudomonas putida
sodium cholate
-
 Pseudomonas putida
Triton X-100
-
 Pseudomonas putida
Tween-20
-
 Pseudomonas putida
Tween-80
-
 Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Pseudomonas putida 5622
-
PHA granule
-
 Pseudomonas putida 70088
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information for optimal activity, it is important to maintain a high ionic strength (300mM NaCl) in the reaction mixture Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q5Y152
-
-
Pseudomonas putida KT 2442 Q5Y152
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,3-tributyryl-sn-glycerol + H2O
-
 Pseudomonas putida ?
-
 ?
1,2,3-tributyryl-sn-glycerol + H2O
-
 Pseudomonas putida KT 2442 ?
-
 ?
additional information hydrolyzes specifically mcl-PHAs containing aliphatic and aromatic monomers, e.g. P(HO-co-HX)n (HO: 87%, HX: 13%), P(HO-co-HX)s (HO: 87%, HX: 13%), PHPhh, PHPhO, P(HN-co-HP) (HN: 55%, HP: 45%) Pseudomonas putida ?
-
 ?
additional information hydrolyzes specifically mcl-PHAs containing aliphatic and aromatic monomers, e.g. P(HO-co-HX)n (HO: 87%, HX: 13%), P(HO-co-HX)s (HO: 87%, HX: 13%), PHPhh, PHPhO, P(HN-co-HP) (HN: 55%, HP: 45%) Pseudomonas putida KT 2442 ?
-
 ?
poly(3-hydroxyphenylheptanoate) + H2O
-
 Pseudomonas putida ?
-
 ?
poly(3-hydroxyphenylheptanoate) + H2O
-
 Pseudomonas putida KT 2442 ?
-
 ?
poly(3-hydroxyphenyloctanoate) + H2O
-
 Pseudomonas putida ?
-
 ?
poly(3-hydroxyphenyloctanoate) + H2O
-
 Pseudomonas putida KT 2442 ?
-
 ?

Synonyms

Synonyms Comment Organism
medium chain length polyhydroxyalkanoate depolymerase
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 very unstable above Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
 Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
7 10.5 pH 7.0: about 25% of maximal activity, pH 10.5: about 50% of maximal activity Pseudomonas putida