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Literature summary for 3.1.1.76 extracted from
- Polyhydroxyalkanoate degrading hydrolase-like activities by Pseudomonas sp. isolated from soil (2004), Int. Biodeter. Biodegrad., 53, 103-109.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DTT | 31% inhibition at 0.05 mM | Pseudomonas fluorescens |  |
| DTT | 38% inhibition at 0.05 mM | Pseudomonas putida | |
| EDTA | 25% inhibition at 0.05 mM | Pseudomonas aeruginosa | |
| KCN | 13% inhibition at 0.005 mM | Pseudomonas aeruginosa | |
| KCN | 14% inhibition at 0.005 mM | Pseudomonas putida | |
| additional information | no inhibition by DTT | Pseudomonas aeruginosa | |
| additional information | no inhibition by KCN, PMSF, sodium azide, EDTA, and sodium deoxycholate | Pseudomonas fluorescens | |
| additional information | no inhibition by sodium azide, EDTA, and sodium deoxycholate | Pseudomonas putida | |
| PMSF | 23% inhibition at 0.05 mM | Pseudomonas aeruginosa | |
| PMSF | 33% inhibition at 0.05 mM | Pseudomonas putida | |
| Sodium azide | 30% inhibition at 0.05 mM | Pseudomonas aeruginosa | |
| sodium deoxycholate | 35% inhibition at 0.5 mM | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
poly(3-hydroxybutyrate) | pH 7.9, 30°C | Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates at 1 mM | Pseudomonas aeruginosa | |
| K+ | stimulates at 1 mM | Pseudomonas aeruginosa | |
| Mg2+ | stimulates at 1 mM | Pseudomonas aeruginosa | |
| additional information | the enzyme requires divalent metal cations for activity | Pseudomonas fluorescens | |
| additional information | the enzyme requires divalent metal cations for activity | Pseudomonas aeruginosa | |
| additional information | the enzyme requires divalent metal cations for activity | Pseudomonas putida | |
| Na+ | stimulates at 1 mM | Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(3-hydroxybutyrate) + H2O | Pseudomonas fluorescens | - |
oligo(3-hydroxybutyrate) | - |
? | |
| poly(3-hydroxybutyrate) + H2O | Pseudomonas aeruginosa | - |
oligo(3-hydroxybutyrate) | - |
? | |
| poly(3-hydroxybutyrate) + H2O | Pseudomonas putida | - |
oligo(3-hydroxybutyrate) | - |
? | |
| poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Pseudomonas fluorescens | co-polymeric substrate | ? | - |
? | |
| poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Pseudomonas aeruginosa | co-polymeric substrate | ? | - |
? | |
| poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Pseudomonas putida | co-polymeric substrate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
isolated from fuel-oil contaminated soil | - |
| Pseudomonas fluorescens | - |
isolated from fuel-oil contaminated soil | - |
| Pseudomonas putida | - |
isolated from fuel-oil contaminated soil | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas fluorescens |
| additional information | - |
- |
Pseudomonas aeruginosa |
| additional information | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(3-hydroxybutyrate) + H2O | - |
Pseudomonas fluorescens | oligo(3-hydroxybutyrate) | - |
? | |
| poly(3-hydroxybutyrate) + H2O | - |
Pseudomonas aeruginosa | oligo(3-hydroxybutyrate) | - |
? | |
| poly(3-hydroxybutyrate) + H2O | - |
Pseudomonas putida | oligo(3-hydroxybutyrate) | - |
? | |
| poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | co-polymeric substrate | Pseudomonas fluorescens | ? | - |
? | |
| poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | co-polymeric substrate | Pseudomonas aeruginosa | ? | - |
? | |
| poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | co-polymeric substrate | Pseudomonas putida | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas fluorescens |
| 30 | - |
assay at | Pseudomonas aeruginosa |
| 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Pseudomonas fluorescens |
| 7.9 | - |
assay at | Pseudomonas aeruginosa |
| 7.9 | - |
assay at | Pseudomonas putida |
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