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Literature summary for 3.1.1.76 extracted from

  • Kim, H.J.; Kim, D.Y.; Nam, J.S.; Bae, K.S.; Rhee, Y.H.
    Characterization of an extracellular medium-chain-length poly(3-hydroxyalkanoate) depolymerase from Streptomyces sp. KJ-72 (2003), Antonie van Leeuwenhoek, 83, 183-189.
    View publication on PubMed
Search for more literature for 3.1.1.76

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme activity is not affected by DTT Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
Acetic anhydride 45% inhibition at 5 mM Streptomyces sp.
diisopropyl fluorophosphate 83% inhibition at 5 mM Streptomyces sp.
additional information no inhibition by cyanamide, iodoacetamide, PMSF, sodium azide, EDTA, and 4-hydroxymercuribenzoate, the enzyme activity is not affected by DTT Streptomyces sp.
N-bromosuccimide 45% inhibition at 1 mM Streptomyces sp.
Triton X-100 92% inhibition at 0.5 mM Streptomyces sp.
Tween 80 58% inhibition at 0.5 mM Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Streptomyces sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27160
-
 MALDI-TOF/mass spectrometry Streptomyces sp.
27164
-
 1 * 28000, SDS-PAGE, 1 * 27164, MALDI-TOF/mass spectrometry Streptomyces sp.
28000
-
 1 * 28000, SDS-PAGE, 1 * 27164, MALDI-TOF/mass spectrometry Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. KJ-72
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Streptomyces sp.

Purification (Commentary)

Purification (Comment) Organism
12.6fold to homogeneity by DEAE ion exchange chromatography and gel filtration Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.7
-
 purified enzyme Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows no lipase activity, the enzyme is active with several 4-nitrophenyl esters of fatty acids Streptomyces sp. ?
-
 ?
additional information the enzyme shows no lipase activity, the enzyme is active with several 4-nitrophenyl esters of fatty acids Streptomyces sp. KJ-72 ?
-
 ?
poly(3-hydroxyheptanoate) + H2O
-
 Streptomyces sp. di(3-hydroxyheptanoate) main product, small amount of monomeric product, no oligomers, identification by GC/MS ?
poly(3-hydroxyheptanoate) + H2O
-
 Streptomyces sp. KJ-72 di(3-hydroxyheptanoate) main product, small amount of monomeric product, no oligomers, identification by GC/MS ?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE, 1 * 27164, MALDI-TOF/mass spectrometry Streptomyces sp.

Synonyms

Synonyms Comment Organism
MCL-PHA depolymerase
-
 Streptomyces sp.
medium-chain-length poly(3-hydroxyalkanoate) depolymerase
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable at Streptomyces sp.
60
-
 1 h, almost complete inactivation Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 70% at pH 7.5 and at pH 9.0 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 fairly stable over this range Streptomyces sp.

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces sp. isoelectric focusing
-
 4.7