Protein Variants | Comment | Organism |
---|---|---|
additional information | the poly((R)-3-hydroxybutyrate)-degrading activities of N285X are reciprocally related to their pNPCn-hydrolyzing activities, with the exception of N285A and N285G, and that His residue could functionally substitute for Asn285 on poly((R)-3-hydroxybutyrate) degradation. Among the mutant enzymes, N285E displays the highest hydrolysis rate regardless of substrate concentration or carbon chain length. N285A has comparable hydrolysis rates to N285E at low substrate concentrations (0.1-0.4 mM) for all the substrates regardless of carbon chain lengths. N285H shows one of the lowest hydrolysis rates among the mutant enzymes regardless of substrate concentration or carbon chain length | Ralstonia pickettii |
N285A | site-directed mutagenesis, mutation at position 285 in the C-terminal domain increases the hydrolytic activity for 4-nitrophenyl esters with carbon chain lengths of C2-C6 | Ralstonia pickettii |
N285D | site-directed mutagenesis, mutation at position 285 in the C-terminal domain increases the hydrolytic activity for 4-nitrophenyl esters with carbon chain lengths of C2-C6 | Ralstonia pickettii |
N285E | site-directed mutagenesis, mutation at position 285 in the C-terminal domain increases the hydrolytic activity for 4-nitrophenyl esters with carbon chain lengths of C2-C6 | Ralstonia pickettii |
N285G | site-directed mutagenesis, mutation at position 285 in the C-terminal domain increases the hydrolytic activity for 4-nitrophenyl esters with carbon chain lengths of C2-C6 | Ralstonia pickettii |
N285H | site-directed mutagenesis, mutation at position 285 in the C-terminal domain increases the hydrolytic activity for 4-nitrophenyl esters with carbon chain lengths of C2-C6 | Ralstonia pickettii |
N285Y | site-directed mutagenesis, mutation at position 285 in the C-terminal domain increases the hydrolytic activity for 4-nitrophenyl esters with carbon chain lengths of C2-C6 | Ralstonia pickettii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutant enzymes, overview | Ralstonia pickettii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly((R)-3-hydroxybutyrate) + H2O | Ralstonia pickettii | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ralstonia pickettii | P12625 | - |
- |
Ralstonia pickettii T1 | P12625 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Ralstonia pickettii | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Ralstonia pickettii T1 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Ralstonia pickettii | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Ralstonia pickettii T1 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Ralstonia pickettii | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Ralstonia pickettii T1 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Ralstonia pickettii | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Ralstonia pickettii T1 | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl valerate + H2O | - |
Ralstonia pickettii | 4-nitrophenol + valerate | - |
? | |
4-nitrophenyl valerate + H2O | - |
Ralstonia pickettii T1 | 4-nitrophenol + valerate | - |
? | |
poly((R)-3-hydroxybutyrate) + H2O | - |
Ralstonia pickettii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PhaZRpiT1 | - |
Ralstonia pickettii |
PHB depolymerase | - |
Ralstonia pickettii |
poly[(R)-3-hydroxybutyrate] depolymerase | - |
Ralstonia pickettii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ralstonia pickettii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Ralstonia pickettii |