Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.74 extracted from

  • Numoto, N.; Kamiya, N.; Bekker, G.J.; Yamagami, Y.; Inaba, S.; Ishii, K.; Uchiyama, S.; Kawai, F.; Ito, N.; Oda, M.
    Structural dynamics of the PET-degrading cutinase-like enzyme from Saccharomonospora viridis AHK190 in substrate-bound states elucidates the Ca2+-driven catalytic cycle (2018), Biochemistry, 57, 5289-5300 .
    View publication on PubMed
Search for more literature for 3.1.1.74

Cloned(Commentary)

Cloned (Comment) Organism
S226P/R228S/S176A with an N-terminal polyhistidine tag is overexpressed in Escherichia coli Saccharomonospora viridis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method at 20°C, crystal structure of the inactive form of a Cut190 mutant, S226P/R228S/S176A, in complex with calcium ions and/or substrates Saccharomonospora viridis

Protein Variants

Protein Variants Comment Organism
S226P/R228S increase in PET degradation, improved activity and thermostability Saccharomonospora viridis
S226P/R228S/S176A inactive mutant enzyme Saccharomonospora viridis

General Stability

General Stability Organism
stability is regulated by Ca2+ binding. One Ca2+ binds to the enzyme, which induces large conformational changes in several loop regions to stabilize an open conformation Saccharomonospora viridis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ three calcium ions bind to S226P/R228S/S176A. Ca2+ binding induces the pocket to open, enabling the substrate to access the pocket more easily. Molecular dynamics simulations suggest that a postreaction state in the engaged form presumably exists between the experimentally observed forms, indicating that the substrate would be cleaved in the engaged form and then requires the enzyme to change to the open form to release the product, a process that Ca2+ can greatly accelerate Saccharomonospora viridis

Organism

Organism UniProt Comment Textmining
Saccharomonospora viridis W0TJ64
-
-
Saccharomonospora viridis AHK190 W0TJ64
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomonospora viridis

Synonyms

Synonyms Comment Organism
Cut190
-
 Saccharomonospora viridis