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Literature summary for 3.1.1.74 extracted from

  • Bermudez-Garcia, E.; Pena-Montes, C.; Castro-Rodriguez, J.A.; Gonzalez-Canto, A.; Navarro-Ocana, A.; Farres, A.
    ANCUT2, a thermo-alkaline cutinase from Aspergillus nidulans and its potential applications (2017), Appl. Biochem. Biotechnol., 182, 1014-1036 .
    View publication on PubMed
Search for more literature for 3.1.1.74

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, 47% residual acivity Aspergillus nidulans
Cu2+ 10 mM, 52% residual acivity Aspergillus nidulans
EDTA 10 mM, 42% residual acivity Aspergillus nidulans
Fe3+ 10 mM, 43% residual acivity Aspergillus nidulans
K+ 1 mM, 42% residual acivity Aspergillus nidulans
Mg2+ 1 mM, 42% residual acivity Aspergillus nidulans
Na+ 1 mM, 39% residual acivity Aspergillus nidulans
PMSF 10 mM, 47% residual acivity Aspergillus nidulans
SDS 10 mM, 47% residual acivity Aspergillus nidulans
Tween 80 10 mM, 48% residual acivity Aspergillus nidulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.88
-
 4-nitrophenyl acetate pH 9.0, 37°C Aspergillus nidulans

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 30%, 44% residual activity Aspergillus nidulans
DMSO 30%, 53% residual activity Aspergillus nidulans
Ethanol 30%, 45% residual activity Aspergillus nidulans
hexane 30%, 53% residual activity Aspergillus nidulans
isopropanol 30%, 46% residual activity Aspergillus nidulans

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans Q5AVY9
-
-
Aspergillus nidulans ATCC 38163 Q5AVY9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Aspergillus nidulans 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Aspergillus nidulans ATCC 38163 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butanoate + H2O about 55% of the activity with 4-nitrophenyl acetate Aspergillus nidulans 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl butanoate + H2O about 55% of the activity with 4-nitrophenyl acetate Aspergillus nidulans ATCC 38163 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl myristate + H2O about 50% of the activity with 4-nitrophenyl acetate Aspergillus nidulans 4-nitrophenol + myristate
-
 ?
4-nitrophenyl myristate + H2O about 50% of the activity with 4-nitrophenyl acetate Aspergillus nidulans ATCC 38163 4-nitrophenol + myristate
-
 ?
4-nitrophenyl palmitate + H2O about 30% of the activity with 4-nitrophenyl acetate Aspergillus nidulans 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl palmitate + H2O about 30% of the activity with 4-nitrophenyl acetate Aspergillus nidulans ATCC 38163 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl stearate + H2O about 28% of the activity with 4-nitrophenyl acetate Aspergillus nidulans 4-nitrophenol + stearate
-
 ?
cutin + H2O
-
 Aspergillus nidulans palmitate + ?
-
 ?
additional information enzyme preferably hydrolyzes short-chain length esters (C2-C4), but it also displays a slight affinity for long-chain length esters (C14-C18) Aspergillus nidulans ?
-
 ?
additional information enzyme preferably hydrolyzes short-chain length esters (C2-C4), but it also displays a slight affinity for long-chain length esters (C14-C18) Aspergillus nidulans ATCC 38163 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE, x * 24350, calculated from sequence, mature protein Aspergillus nidulans

Synonyms

Synonyms Comment Organism
Cut2
-
 Aspergillus nidulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus nidulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 half-life 407 min Aspergillus nidulans
50
-
 half-life 110 min Aspergillus nidulans
60
-
 1h, pH 6-10, 60% residual activity Aspergillus nidulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
40.3
-
 4-nitrophenyl acetate pH 9.0, 37°C Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Aspergillus nidulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 1h, 60°C, 60% residual activity Aspergillus nidulans

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus nidulans calculated from sequence
-
 4.9
Aspergillus nidulans isoelectric focusing
-
 5

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.87
-
 4-nitrophenyl acetate pH 9.0, 37°C Aspergillus nidulans