Cloned (Comment) | Organism |
---|---|
- |
Trichoderma reesei |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor diffusion method, the structure of a cutinase in native and inhibitor-bound conformations is reported | Trichoderma reesei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
butyl 4-nitrophenyl undecylphosphonate | in the absence of surfactant, the rate of cutinase inhibition is very low. The addition of beta-octylglucoside is required to trigger the inhibition of cutinase, which is completely inactivated after 60 min | Trichoderma reesei | |
E600 | in the absence of surfactant, no inhibition is observed with E600. The addition of beta-octylglucoside is required to trigger the inhibition of cutinase, which is completely inactivated after 12 min | Trichoderma reesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23748 | - |
matrix-assisted laser desorption-ionization/time-of-flight mass spectrometry | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | G0RH85 | - |
- |
Trichoderma reesei QM6a | G0RH85 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | cleavage of a propeptide | Trichoderma reesei |
Purification (Comment) | Organism |
---|---|
- |
Trichoderma reesei |
Renatured (Comment) | Organism |
---|---|
after unfolding cutinase at 80°C, cooling at 20°C restores the initial conformation | Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cutin + H2O | apple cutin | Trichoderma reesei | cutin monomers | - |
? | |
cutin + H2O | apple cutin | Trichoderma reesei QM6a | cutin monomers | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 5.0, 20 h, less than 20% loss of activity | Trichoderma reesei |
60 | - |
pH 5.0, 1 h, less than 20% loss of activity | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
the enzyme exhibits two local pH optima, one at pH 4.0 and the other one at pH 7.3 | Trichoderma reesei |
7.3 | - |
the enzyme exhibits two local pH optima, one at pH 4.0 and the other one at pH 7.3 | Trichoderma reesei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | active over a broad pH range | Trichoderma reesei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | over 80% of the initial activity is retained between pH 4.0 and pH 7.4 after 20 h at 50°C | Trichoderma reesei |