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Literature summary for 3.1.1.74 extracted from
- Novel cutinase from Pseudomonas cepacia NRRL B 2320: purification, characterization and identification of cutinase encoding genes (2013), J. Gen. Appl. Microbiol., 59, 171-184.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| two genes, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3), production and assay method optimization, overview | Burkholderia cepacia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | slight inhibition at 10 mM | Burkholderia cepacia |  |
| acetone | 92% inhibition at 75% v/v | Burkholderia cepacia | |
| Cu2+ | 85% inhibition at 1 mM | Burkholderia cepacia | |
| Dichloromethane | 78% inhibition at 75% v/v | Burkholderia cepacia | |
| dioxan | 23% inhibition at 75% v/v | Burkholderia cepacia | |
| DMSO | 49% inhibition at 75% v/v | Burkholderia cepacia | |
| ethanol | 97% inhibition at 75% v/v | Burkholderia cepacia | |
| Fe2+ | 20% inhibition at 1 mM | Burkholderia cepacia | |
| Hg2+ | inhibits completely at 1 mM | Burkholderia cepacia | |
| Isopropanol | complete inhibition at 75% v/v | Burkholderia cepacia | |
| methanol | 90% inhibition at 75% v/v | Burkholderia cepacia | |
| additional information | no inhibition by EDTA, Triton X-100 and Tween 80, or by 75% v/v chloroform, isooctane, isoamyl alcohol, or butanol | Burkholderia cepacia | |
| n-hexane | 18.5% inhibition at 75% v/v | Burkholderia cepacia | |
| PMSF | 95% inhibition at 1 mM | Burkholderia cepacia | |
| SDS | - |
Burkholderia cepacia | |
| sodium deoxycholate | - |
Burkholderia cepacia | |
| Zn2+ | 18% inhibition at 1 mM | Burkholderia cepacia | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetic analysis | Burkholderia cepacia |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Burkholderia cepacia | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates at 1 mM | Burkholderia cepacia | |
| Co2+ | activates at 1 mM | Burkholderia cepacia | |
| K+ | activates at 1 mM | Burkholderia cepacia | |
| Mg2+ | activates at 1 mM | Burkholderia cepacia | |
| additional information | Ni2+ and Mn2+ do not affect enzyme activity at 1 mM | Burkholderia cepacia | |
| Na+ | activates at 1 mM | Burkholderia cepacia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26250 | - |
x * 26250, mass spectrometry, x * 26500, recombinant enzyme, SDS-PAGE | Burkholderia cepacia |
| 26500 | - |
x * 26250, mass spectrometry, x * 26500, recombinant enzyme, SDS-PAGE | Burkholderia cepacia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cutin + H2O | Burkholderia cepacia | - |
cutin monomers | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | - |
- |
- |
| Burkholderia cepacia NRRL B 2320 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 1.42fold to homogeneity by ammoium sulfate fractionation, cation exchange chromatography, and two steps of gel filtration | Burkholderia cepacia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5359 | - |
substrate 4-nitrophenyl butyrate, recombinant enzyme, pH 8.0, 37°C | Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl (16-methyl sulfonyl ester) hexadecanoate + H2O | - |
Burkholderia cepacia | 4-nitrophenol + (16-methyl sulfonyl ester) hexadecanoate | - |
? | |
| 4-nitrophenyl butyrate + H2O | best 4-nitrophenyl ester substrate | Burkholderia cepacia | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | best 4-nitrophenyl ester substrate | Burkholderia cepacia NRRL B 2320 | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl caprylate + H2O | - |
Burkholderia cepacia | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl caprylate + H2O | - |
Burkholderia cepacia NRRL B 2320 | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl laurate + H2O | low activity | Burkholderia cepacia | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl palmitate + H2O | low activity | Burkholderia cepacia | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | low activity | Burkholderia cepacia NRRL B 2320 | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl valerate + H2O | - |
Burkholderia cepacia | 4-nitrophenol + valerate | - |
? | |
| 4-nitrophenyl valerate + H2O | - |
Burkholderia cepacia NRRL B 2320 | 4-nitrophenol + valerate | - |
? | |
| cutin + H2O | - |
Burkholderia cepacia | cutin monomers | - |
? | |
| additional information | the enzyme prefers 4-nitrophenyl ester substrates with chain lengths of C4-C6, production and assay method optimization, overview | Burkholderia cepacia | ? | - |
? | |
| additional information | the enzyme prefers 4-nitrophenyl ester substrates with chain lengths of C4-C6, production and assay method optimization, overview | Burkholderia cepacia NRRL B 2320 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26250, mass spectrometry, x * 26500, recombinant enzyme, SDS-PAGE | Burkholderia cepacia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36.5 | - |
- |
Burkholderia cepacia |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | activity range, inactive above | Burkholderia cepacia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
first order rate constants in thermal inactivation, overview | Burkholderia cepacia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
- |
Burkholderia cepacia |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | activity range, inactive below | Burkholderia cepacia |
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Release 2023.1 (January 2023)
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