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Literature summary for 3.1.1.74 extracted from
- Biochemical characterization of the cutinases from Thermobifida fusca (2010), J. Mol. Catal. B, 63, 121-127.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of cutinase FspC in Bacillus subtilis | Fusarium vanettenii |
| expression of cutinase isozymes Tfu 0882 and Tfu 0883 in Escherichia coli strain BL21(DE3) | Thermobifida fusca |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | inhibits isozyme Tfu 0882 7% | Thermobifida fusca |  |
| Ca2+ | inhibits 6% at 1 mM | Fusarium vanettenii | |
| Ca2+ | inhibits isozyme Tfu 0882 28% and isozyme Tfu 0883 18% at 1 mM | Thermobifida fusca | |
| Cr3+ | inhibits 72% at 1 mM | Fusarium vanettenii | |
| Cr3+ | inhibits isozyme Tfu 0882 89% and isozyme Tfu 0883 90% at 1 mM | Thermobifida fusca | |
| Cu2+ | inhibits 17% at 1 mM | Fusarium vanettenii | |
| Fe2+ | inhibits 54% at 1 mM | Fusarium vanettenii | |
| Fe2+ | inhibits isozyme Tfu 0882 45% and isozyme Tfu 0883 36% at 1 mM | Thermobifida fusca | |
| Hg2+ | inhibits completely at 1 mM | Fusarium vanettenii | |
| Hg2+ | inhibits isozyme Tfu 0882 and isozyme Tfu 0883 completely at 1 mM | Thermobifida fusca | |
| Mg2+ | inhibits 11% at 1 mM | Fusarium vanettenii | |
| Mg2+ | inhibits isozyme Tfu 0882 34% | Thermobifida fusca | |
| Ni2+ | inhibits 7% at 1 mM | Thermobifida fusca | |
| Pb2+ | inhibits 39% at 1 mM | Fusarium vanettenii | |
| Pb2+ | inhibits isozyme Tfu 0882 48% and isozyme Tfu 0883 52% at 1 mM | Thermobifida fusca | |
| Zn2+ | inhibits 18% at 1 mM | Fusarium vanettenii | |
| Zn2+ | inhibits isozyme Tfu 0882 50% and isozyme Tfu 0883 56% at 1 mM | Thermobifida fusca | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.272 | - |
4-nitrophenyl butyrate | pH 8.0, 60°C, recombinant cutinase FspC | Fusarium vanettenii | |
| 0.505 | - |
4-nitrophenyl butyrate | pH 8.0, 60°C, isozyme Tfu 0883 | Thermobifida fusca | |
| 0.673 | - |
4-nitrophenyl butyrate | pH 8.0, 60°C, isozyme Tfu 0882 | Thermobifida fusca | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | activates isozyme Tfu 0883 7% at 1 mM | Thermobifida fusca | |
| Co2+ | activates 7% at 1 mM | Fusarium vanettenii | |
| Co2+ | activates isozyme Tfu 0882 4% and isozyme Tfu 0883 24% at 1 mM | Thermobifida fusca | |
| Mg2+ | activates isozyme Tfu 0883 11% at 1 mM | Thermobifida fusca | |
| Mn2+ | activates 36% at 1 mM | Fusarium vanettenii | |
| Mn2+ | activates isozyme Tfu 0882 9% and isozyme Tfu 0883 24% at 1 mM | Thermobifida fusca | |
| additional information | Ba2+ and EDTA have no effect on enzyme activity at 1 mM and 10 mM, respectively | Thermobifida fusca | |
| additional information | EDTA has no effect on enzyme activity at 10 mM | Fusarium vanettenii | |
| Ni2+ | activates isozyme Tfu 0882 5% and isozyme Tfu 0883 7% at 1 mM | Fusarium vanettenii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28997 | - |
1 * 29000, isozymes Tfu 0882 and Tfu 0883, SDS-PAGE, 1 * 29220, isozyme Tfu 0882, sequence calculation, 1 * 28997, isozyme Tfu 0883, sequence calculation | Thermobifida fusca |
| 29000 | - |
1 * 29000, isozymes Tfu 0882 and Tfu 0883, SDS-PAGE, 1 * 29220, isozyme Tfu 0882, sequence calculation, 1 * 28997, isozyme Tfu 0883, sequence calculation | Thermobifida fusca |
| 29220 | - |
1 * 29000, isozymes Tfu 0882 and Tfu 0883, SDS-PAGE, 1 * 29220, isozyme Tfu 0882, sequence calculation, 1 * 28997, isozyme Tfu 0883, sequence calculation | Thermobifida fusca |
| 32000 | - |
recombinant isozymes Tfu 0882 and Tfu 0883, gel filtration | Thermobifida fusca |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Thermobifida fusca | the enzyme exhibits a broad substrate specificity against plant cutin, synthetic polyesters, insoluble triglycerides, and soluble esters | ? | - |
? | |
| additional information | Fusarium vanettenii | the enzyme exhibits a broad substrate specificity against plant cutin, synthetic polyesters, insoluble triglycerides, and soluble esters | ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | stability of the cutinase in different organic solvents. The cutinase is incubated with 75% v/v of organic solvent in assay buffer at 20°C for 18 h, overview | Fusarium vanettenii |
| additional information | stability of the cutinase in different organic solvents. The cutinase isozymes are incubated with 75% v/v of organic solvent in assay buffer at 20°C for 18 h, overview | Thermobifida fusca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium vanettenii | - |
- |
- |
| Thermobifida fusca | - |
two isozymes Tfu 0882 and Tfu 0883 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant cutinase FspC from Bacillus subtilis | Fusarium vanettenii |
| recombinant cutinase isozymes Tfu 0882 and Tfu 0883 from Escherichia coli strain BL21(DE3) | Thermobifida fusca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | - |
Thermobifida fusca | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Fusarium vanettenii | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Thermobifida fusca | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Fusarium vanettenii | 4-nitrophenol + palmitate | - |
? | |
| additional information | the enzyme exhibits a broad substrate specificity against plant cutin, synthetic polyesters, insoluble triglycerides, and soluble esters | Thermobifida fusca | ? | - |
? | |
| additional information | the enzyme exhibits a broad substrate specificity against plant cutin, synthetic polyesters, insoluble triglycerides, and soluble esters | Fusarium vanettenii | ? | - |
? | |
| tributyrin + H2O | - |
Thermobifida fusca | ? | - |
? | |
| tributyrin + H2O | - |
Fusarium vanettenii | ? | - |
? | |
| triolein + H2O | - |
Thermobifida fusca | ? | - |
? | |
| triolein + H2O | - |
Fusarium vanettenii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Fusarium vanettenii |
| monomer | 1 * 29000, isozymes Tfu 0882 and Tfu 0883, SDS-PAGE, 1 * 29220, isozyme Tfu 0882, sequence calculation, 1 * 28997, isozyme Tfu 0883, sequence calculation | Thermobifida fusca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FspC | - |
Fusarium vanettenii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Fusarium vanettenii |
| 60 | - |
- |
Thermobifida fusca |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 483 | - |
4-nitrophenyl butyrate | pH 8.0, 60°C, isozyme Tfu 0882 | Thermobifida fusca | |
| 742 | - |
4-nitrophenyl butyrate | pH 8.0, 60°C, isozyme Tfu 0883 | Thermobifida fusca | |
| 837 | - |
4-nitrophenyl butyrate | pH 8.0, 60°C, recombinant cutinase FspC | Fusarium vanettenii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Thermobifida fusca |
| 8 | - |
assay at | Fusarium vanettenii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 150 | - |
4-nitrophenyl butyrate | pH 8.0, 60°C, isozyme Tfu 0883 | Thermobifida fusca | |
| 700 | - |
4-nitrophenyl butyrate | pH 8.0, 60°C, isozyme Tfu 0882 | Thermobifida fusca | |
| 3200 | - |
4-nitrophenyl butyrate | pH 8.0, 60°C, recombinant cutinase FspC | Fusarium vanettenii | |
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