Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.74 extracted from

  • Badenes, S.; Lemos, F.; Cabral, J.
    Stability of cutinase, wild type and mutants, in AOT reversed micellar system-effect of mixture components of alkyl esters production (2011), J. Chem. Technol. Biotechnol., 86, 34-41.
No PubMed abstract available
Search for more literature for 3.1.1.74

Application

Application Comment Organism
synthesis recombinant cutinase from Fusarium solani pisi is used as a catalyst in enzymatic transesterification between a mixture of triglyceride oils and methanol for biodiesel production in a bis(2-ethylhexyl) sodium sulfosuccinate (AOT)/isooctane reversed micellar system, kinetics, overview Fusarium vanettenii

Protein Variants

Protein Variants Comment Organism
L153Q site-directed mutagenesis, the mutant shows transesterification activity similar to the wild-type enzyme Fusarium vanettenii
additional information cutinase is microencapsulated in reversed micelles of bis(2-ethylhexyl) sodium sulfosuccinate in isooctane for the production of alkyl esters, known as biodiesel, evaluation of the system stability using wild-type enzyme and three mutants, L153Q, T179C and S54D, method evaluation, overview. Loss of 45% of wild-type cutinase activity when incubated in the micellar system for 3 h, and an additional loss of 90% of the activity is observed in the presence of methanol after 10 min of incubation Fusarium vanettenii
S54D site-directed mutagenesis, the mutant shows reduced transesterification activity compared to the wild-type enzyme Fusarium vanettenii
T179C site-directed mutagenesis, the mutant shows transesterification activity similar to the wild-type enzyme, T179C displays high stability in the presence of methanol with an activity loss of only 16% as compared to 90% loss of wild-type activity, the mutant is also more stable microencapsulated in reversed micelles of bis(2-ethylhexyl) sodium sulfosuccinate in isooctane Fusarium vanettenii

Inhibitors

Inhibitors Comment Organism Structure
glycerol inhibits the transesterification activity of the cutinase after 10 min of incubation Fusarium vanettenii

Organism

Organism UniProt Comment Textmining
Fusarium vanettenii
-
-
-