Application | Comment | Organism |
---|---|---|
synthesis | application of enginered mutant T101A/Q132A/I218A in synthetic fiber biotransformation | Thermobifida fusca |
Protein Variants | Comment | Organism |
---|---|---|
I218A | engineering by site-directed mutagenesis modifying the active site, the mutant cutinase shows increased activity on polyester substrates. Mutation I218A creates space, activity on poly(ethylene terephthalate) is increased compared to the wild-type enzyme, with considerably higher hydrolysis efficiency | Thermobifida fusca |
T101A/Q132A | engineering by site-directed mutagenesis modifying the active site, the mutant cutinase shows increased activity on polyester substrates. The double mutation Q132A/T101A both creates space and increases hydrophobicity. The activity of the double mutant on the soluble substrate p-nitrophenyl butyrate increased 2fold compared to wild-type cutinase, while on poly(ethylene terephthalate) the double mutant exhibits considerably higher hydrolysis efficiency | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
Tfu_0883 | - |
Thermobifida fusca |