Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | Pichia pastoris expressing the native non-tagged cutinase exhibits about 2- and 3fold higher values of protein amount and cutinase activity in the culture supernatant, respectively, than those containing the C-terminal tagged cutinase | Fusarium solani |
Application | Comment | Organism |
---|---|---|
biotechnology | high-level secretion of cutinase in Pichia pastoris may be a promising alternative to many expression systems previously used for the large-scale production of cutinase in Saccharomyces cerevisiae as well as Escherichia coli. The functional expression of a large amount of extracellular cutinase offers the opportunity for developing an efficient high-throughput screening procedure for the improvement of enzymatic property and the development of novel biocatalysis of cutinase | Fusarium solani |
Cloned (Comment) | Organism |
---|---|
into pPICZalphaA with the Saccharomyces cerevisiae alpha-factor signal sequence and methanol-inducible alcohol oxidase promoter, additional sequences of the c-myc epitope and (His)6-tag of the vector fused to the C-terminus of cutinase, while the other expression vector is constructed without any additional sequence. Expressed in Pichia pastoris strain X-33 | Fusarium solani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium solani | P00590 | - |
- |
Purification (Comment) | Organism |
---|---|
non-tagged and the C-terminal tagged cutinases, by diafiltration | Fusarium solani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tributyrin + H2O | - |
Fusarium solani | ? | - |
? | |
tricaprylin + H2O | - |
Fusarium solani | ? | - |
? | |
triolein + H2O | - |
Fusarium solani | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cutinase | - |
Fusarium solani |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 45 | non-tagged and the C-terminal tagged cutinases | Fusarium solani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | non-tagged and the C-terminal tagged cutinases | Fusarium solani |