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Literature summary for 3.1.1.74 extracted from

  • Takahashi, K.; Shimada, T.; Kondo, M.; Tamai, A.; Mori, M.; Nishimura, M.; Hara-Nishimura, I.
    Ectopic expression of an esterase, which is a candidate for the unidentified plant cutinase, causes cuticular defects in Arabidopsis thaliana (2010), Plant Cell Physiol., 51, 123-131.
    View publication on PubMed
Search for more literature for 3.1.1.74

Cloned(Commentary)

Cloned (Comment) Organism
transgenic Arabidopsis plants expressing GFP-CDEF1 fusion protein driven by the 35S promoter. CDEF1 expressed with a 6 x His tag at its C-terminus in suspension cultures of BY-2 tobacco cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information CDEF1-deficient mutant (SALK-014093) that carries a T-DNA insertion in the coding region of CDEF1, shows no abnormal phenotypes, such as reduced fertility or reduced lateral root emergence Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39200
-
 sequence analysis Arabidopsis

Organism

Organism UniProt Comment Textmining
Arabidopsis
-
-
-
Arabidopsis thaliana Q9SZW7
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni2+ affinity column Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf CDEF1 is secreted to the extracellular space in leaves Arabidopsis thaliana
-
pollen
-
 Arabidopsis
-
pollen in mature pollen grains within non-dehiscent anthers Arabidopsis thaliana
-
pollen tube pollen tubes that are germinating on the stigma Arabidopsis thaliana
-
root at the zone of lateral root emergence Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45
-
 at 30°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
 Arabidopsis thaliana 4-nitrophenol + butyrate
-
 ?

Subunits

Subunits Comment Organism
? x * 39200, calculated from sequence Arabidopsis

Synonyms

Synonyms Comment Organism
CDEF1
-
 Arabidopsis
CDEF1
-
 Arabidopsis thaliana
cuticle destructing factor 1
-
 Arabidopsis
cuticle destructing factor 1
-
 Arabidopsis thaliana
cutinase
-
 Arabidopsis
cutinase
-
 Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 activity is reduced to less than 10% of the maximum activity by heating for 10 min at 99°C Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana CDEF1 gene is not expressed in leaves of wild-type plants. Is expressed at low levels in roots down
Arabidopsis thaliana CDEF1 gene is expressed in leaves of transgenic 35S::CDEF1 plants. CDEF1 gene is highly expressed in mature pollen up

General Information

General Information Comment Organism
physiological function CDEF1 is a plant cutinase, recombinant CDEF1 protein has esterase activity. Ectopic expression of CDEF1 driven by the 35S promoter causes fusion of organs, including leaves, stems and flowers, and increased surface permeability. CDEF1 is involved in the penetration of the stigma by pollen tubes. CDEF1 degrades cell wall components to facilitate the emergence of the lateral roots Arabidopsis thaliana
physiological function ectopic expression of CDEF1 driven by the 35S promoter causes fusion of organs, including leaves, stems and flowers, and increased surface permeability Arabidopsis