Literature summary for 3.1.1.74 extracted from

Rocha, A.L.; Di Pietro, A.; Ruiz-Roldan, C.; Roncero, M.I.
Ctf1, a transcriptional activator of cutinase and lipase genes in Fusarium oxysporum is dispensable for virulence (2008), Mol. Plant Pathol., 9, 293-304.

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Cloned(Commentary)

Cloned (Comment)	Organism
amplified DNA fragments cloned into the pGEM-T vector, cut1 isolated from a lambdaEMBL3 genomic library	Fusarium oxysporum f. sp. lycopersici

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21700	-	calculated from sequence	Fusarium oxysporum f. sp. lycopersici

Organism

Organism	UniProt	Comment	Textmining
Fusarium oxysporum f. sp. lycopersici	A6N6J6	-	-
Fusarium oxysporum f. sp. lycopersici 4287	A6N6J6	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	microconidium	Fusarium oxysporum f. sp. lycopersici	-
mycelium	-	Fusarium oxysporum f. sp. lycopersici	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl butyrate + H2O	-	Fusarium oxysporum f. sp. lycopersici	4-nitrophenol + butyrate	-	?
4-nitrophenyl butyrate + H2O	-	Fusarium oxysporum f. sp. lycopersici 4287	4-nitrophenol + butyrate	-	?

Synonyms

Synonyms	Comment	Organism
cutinase	-	Fusarium oxysporum f. sp. lycopersici

pI Value

Organism	Comment	pI Value Maximum	pI Value
Fusarium oxysporum f. sp. lycopersici	calculated from sequence	-	8.1

Expression

Organism	Comment	Expression
Fusarium oxysporum f. sp. lycopersici	mutants carrying a DELTActf1 loss-of-function allele grown on inducing substrates fail to activate extracellular cutinolytic activity and expression of the cut1 gene, encoding a putative cutinase	down
Fusarium oxysporum f. sp. lycopersici	the zinc finger transcription factor Ctf1 from Fusarium oxysporum mediates efficient transcriptional activation of cut1 and lip1 on fatty acid substrates, but not on glucose. The Ctf1 transcription factor, a functional orthologue of CTF1alpha, controls expression of cutinase genes and virulence. Strains harbouring a ctf1C allele in which the ctf1 coding region is fused to the strong constitutive Aspergillus nidulans gpdA promoter show increased induction of cutinase activity and gene expression. Higher levels of cut1 transcript in mycelia of the wild-type grown on apple cutin than in those grown on glucose. Activation of cut1 by apple cutin is even more pronounced in the ctf1C strain than in the wild-type. Low expression of cut1 in roots 10 days after inoculation of tomato plants	up

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Release 2023.1 (January 2023)