Literature summary for 3.1.1.74 extracted from

Rubio, M.B.; Cardoza, R.E.; Hermosa, R.; Gutierrez, S.; Monte, E.
Cloning and characterization of the Thcut1 gene encoding a cutinase of Trichoderma harzianum T34 (2008), Curr. Genet., 54, 301-312.

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Activating Compound

Activating Compound	Comment	Organism	Structure
16-hydroxyhexadecanoic acid	is strongly induced in vitro by cutin monomer 16-hydroxyhexadecanoic acid	Trichoderma harzianum
olive oil	Thcut1 mRNA is strongly induced in vitro by olive oil	Trichoderma harzianum

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris	Trichoderma harzianum

Inhibitors

Inhibitors	Comment	Organism	Structure
D-glucose	Thcut1 mRNA is repressed by glucose	Trichoderma harzianum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.085	-	p-nitrophenyl palmitate	-	Trichoderma harzianum
0.33	-	p-nitrophenyl acetate	-	Trichoderma harzianum
0.57	-	p-nitrophenyl butyrate	-	Trichoderma harzianum
0.82	-	p-nitrophenyl valerate	-	Trichoderma harzianum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Trichoderma harzianum	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29000	-	SDS-PAGE	Trichoderma harzianum

Organism

Organism	UniProt	Comment	Textmining
Trichoderma harzianum	A8QPD8	T34	-
Trichoderma harzianum T34	A8QPD8	T34	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.004	-	4-nitrophenyl palmitate, esterase activity of the recombinant THCUT1 protein against different chromogenic substrates	Trichoderma harzianum
0.12	-	in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 1.8, 1.1, and 0.12 micromol/min/mg against 4-nitrophenyl palmitate, at 4, 8 and 24 h	Trichoderma harzianum
0.25	-	4 h cultures against 4-nitrophenyl palmitate	Trichoderma harzianum
0.4	-	4-nitrophenyl valerate, esterase activity of the recombinant THCUT1 protein against different chromogenic substrates	Trichoderma harzianum
0.74	-	4-nitrophenyl butyrate, esterase activity of the recombinant THCUT1 protein against different chromogenic substrates	Trichoderma harzianum
0.85	-	in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 11.4, 5.4, and 0.85 micromol/min/mg against 4-nitrophenyl butyrate, at 4, 8 and 24 h	Trichoderma harzianum
1.1	-	in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 1.8, 1.1, and 0.12 micromol/min/mg against 4-nitrophenyl palmitate, at 4, 8 and 24 h	Trichoderma harzianum
1.2	-	supernatant from 8 h culture on 0.2% cutin monomer 16-hydroxy-hexadecanoic acid shows slightly higher values against 4-nitrophenyl palmitate	Trichoderma harzianum
1.3	-	supernatant from 8 h culture on 0.05% cutin monomer 16-hydroxy-hexadecanoic acid shows slightly higher values against 4-nitrophenyl palmitate	Trichoderma harzianum
1.8	-	in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 1.8, 1.1, and 0.12 micromol/min/mg against 4-nitrophenyl palmitate, at 4, 8 and 24 h	Trichoderma harzianum
2.84	-	4-nitrophenyl acetate, esterase activity of the recombinant THCUT1 protein against different chromogenic substrates	Trichoderma harzianum
4.2	-	4 h cultures against 4-nitrophenyl butyrate (6.6 and 4.2 micromol/min/mg)	Trichoderma harzianum
5.4	-	in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 11.4, 5.4, and 0.85 micromol/min/mg against 4-nitrophenyl butyrate, at 4, 8 and 24 h	Trichoderma harzianum
6.6	-	4 h cultures against 4-nitrophenyl butyrate (6.6 and 4.2 micromol/min/mg)	Trichoderma harzianum
7.5	-	supernatant from 8 h culture on 0.2% cutin monomer 16-hydroxy-hexadecanoic acid shows higher activity values against 4-nitrophenyl butyrate	Trichoderma harzianum
9.8	-	supernatant from 8 h culture on 0.05% cutin monomer 16-hydroxy-hexadecanoic acid shows higher activity values against 4-nitrophenyl butyrate	Trichoderma harzianum
11.4	-	in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 11.4, 5.4, and 0.85 micromol/min/mg against 4-nitrophenyl butyrate, at 4, 8 and 24 h	Trichoderma harzianum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	Trichoderma harzianum	4-nitrophenol + acetate	-	?
4-nitrophenyl acetate + H2O	-	Trichoderma harzianum T34	4-nitrophenol + acetate	-	?
4-nitrophenyl butyrate + H2O	-	Trichoderma harzianum	4-nitrophenol + butyrate	-	?
4-nitrophenyl butyrate + H2O	-	Trichoderma harzianum T34	4-nitrophenol + butyrate	-	?
4-nitrophenyl palmitate + H2O	-	Trichoderma harzianum	4-nitrophenol + palmitate	-	?
4-nitrophenyl palmitate + H2O	-	Trichoderma harzianum T34	4-nitrophenol + palmitate	-	?
4-nitrophenyl valerate + H2O	-	Trichoderma harzianum	4-nitrophenol + pentanoate	-	?
4-nitrophenyl valerate + H2O	-	Trichoderma harzianum T34	4-nitrophenol + pentanoate	-	?
cutin + H2O	-	Trichoderma harzianum	cutin monomers	-	?
p-nitrophenyl valerate + H2O	-	Trichoderma harzianum	p-nitrophenol + pentanoate	-	?
p-nitrophenyl valerate + H2O	-	Trichoderma harzianum T34	p-nitrophenol + pentanoate	-	?

Synonyms

Synonyms	Comment	Organism
cutinase	-	Trichoderma harzianum
Thcut1	-	Trichoderma harzianum
THCUT1 protein	-	Trichoderma harzianum

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Release 2023.1 (January 2023)