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Literature summary for 3.1.1.74 extracted from
- Influence of tryptophan tags on the purification of cutinase, secreted by a recombinant Saccharomyces cerevisiae, using cationic expanded bed adsorption and hydrophobic interaction chromatography (2008), Biotechnol. Lett., 30, 1353-1358.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| cutinase-tryptophan,proline2 | tryptophan tag, cutinase with varying length tryptophan tag (WP)2 | Saccharomyces cerevisiae |
| cutinase-tryptophan,proline4 | tryptophan tag, cutinase with varying length tryptophan tag (WP)4 | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Saccharomyces cerevisiae | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification includes cationic Expanded Bed Adsorption (EBA) followed by hydrophobic interaction chromatography (HIC) | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 40 | - |
cutinase-tryptophan,proline4, after 72 h of Saccharomyces cerevisae cultivation | Saccharomyces cerevisiae |
| 200 | - |
cutinase-tryptophan,proline2, after 72 h of Saccharomyces cerevisae cultivation | Saccharomyces cerevisiae |
| 350 | - |
wild type, after 72 h of Saccharomyces cerevisae cultivation | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cutinase | - |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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