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Literature summary for 3.1.1.74 extracted from
- A novel polyesterase from Coprinopsis cinereus hydrolysing cutin and suberin (2009), Appl. Environ. Microbiol., 75, 2148-2157.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | can be exploited in treating agricultural, food, and forest raw materials as well as their processing by-products | Coprinopsis cinerea |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned and expressed in Trichoderma reesei | Coprinopsis cinerea |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 6982 | - |
N-terminal fragment, electrospray ionization Q-TOF mass spectrometric analysis | Coprinopsis cinerea |
| 12140 | - |
C-terminal fragment, Ala(95) - His(204), electrospray ionization Q-TOF mass spectrometric analysis | Coprinopsis cinerea |
| 12270 | - |
C-terminal fragment, Ala(95) - His(205), electrospray ionization Q-TOF mass spectrometric analysis | Coprinopsis cinerea |
| 18800 | - |
around, electrospray ionization Q-TOF mass spectrometric analysis gives two masses 18726.0 Da and 18863.0 Da | Coprinopsis cinerea |
| 20000 | - |
SDS-PAGE | Coprinopsis cinerea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coprinopsis cinerea | B9U443 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by immobilized metal affinity chromatography exploiting a C-terminal His-tag | Coprinopsis cinerea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cutin + H2O | - |
Coprinopsis cinerea | cutin monomers | - |
? |  |
| additional information | CcCUT1 has higher activity on shorter (C2-C10) 12 fatty acid esters of p-nitrophenol than on longer ones and it also exhibited lipase activity. Microscopical analyses and determination of released hydrolysis products showed that the enzyme is able to depolymerize apple cutin and birch outer bark suberin | Coprinopsis cinerea | ? | - |
? | |
| p-nitrophenyl acetate + H2O | concentration of substrate dispersion is 5 mM | Coprinopsis cinerea | p-nitrophenol + acetate | - |
? | |
| p-nitrophenyl butyrate + H2O | concentration of substrate dispersion is 5 mM | Coprinopsis cinerea | p-nitrophenol + butyrate | - |
? | |
| p-nitrophenyl caprate + H2O | concentration of substrate dispersion is 5 mM | Coprinopsis cinerea | p-nitrophenol + ? | - |
? | |
| p-nitrophenyl caproate + H2O | concentration of substrate dispersion is 5 mM | Coprinopsis cinerea | p-nitrophenol + ? | - |
? | |
| p-nitrophenyl laurate + H2O | concentration of substrate dispersion is 5 mM | Coprinopsis cinerea | p-nitrophenol + ? | - |
? | |
| p-nitrophenyl myristate + H2O | concentration of substrate dispersion is 5 mM | Coprinopsis cinerea | p-nitrophenol + ? | - |
? | |
| p-nitrophenyl palmitate + H2O | concentration of substrate dispersion is 5 mM | Coprinopsis cinerea | p-nitrophenol + ? | - |
? | |
| p-nitrophenyl propionate + H2O | concentration of substrate dispersion is 5 mM | Coprinopsis cinerea | p-nitrophenol + ? | - |
? | |
| p-nitrophenyl stearate + H2O | a lower concentration of p-nitrophenyl stearate (2.5 mM) is used due to its lower solubility | Coprinopsis cinerea | p-nitrophenol + ? | - |
? | |
| p-nitrophenyl valerate + H2O | concentration of substrate dispersion is 5 mM | Coprinopsis cinerea | p-nitrophenol + pentanoate | - |
? | |
| suberin + H2O | 9,10-epoxy-18-hydroxy 5 octadecanoic acid | Coprinopsis cinerea | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CcCUT1 | - |
Coprinopsis cinerea |
| cutinase | - |
Coprinopsis cinerea |
| cutinolytic polyesterase | - |
Coprinopsis cinerea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
activity assay at | Coprinopsis cinerea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
enzyme retains 80% of its activity after 20 h incubation at 50°C, but residual activity decreases sharply at 60°C | Coprinopsis cinerea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
and a second optimum at 8.0, but retains activity over a wide pH range | Coprinopsis cinerea |
| 8 | - |
and a second optimum at 7.0, but retains activity over a wide pH range | Coprinopsis cinerea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
retains activity over a wide pH range | Coprinopsis cinerea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
retains activity over a wide pH range | Coprinopsis cinerea |
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Release 2023.1 (January 2023)
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