General Stability | Organism |
---|---|
ANS binds strongly to native cutinase as a noncompetitive inhibitor with up to 5 ANS per cutinase molecule. The first ANS molecule stabilizes cutinase. The last 4 ANS molecules decrease Tm by up to 7°C | Fusarium solani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ANS | binds strongly to native cutinase as a noncompetitive inhibitor with up to 5 ANS per cutinase molecule. The first ANS molecule stabilizes cutinase. The last 4 ANS molecules decrease Tm by up to 7°C | Fusarium solani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000121 | - |
p-nitrophenyl butyrate | 20°C, pH 8, no additive | Fusarium solani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium solani | P00590 | recombinantly expressed in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl butyrate + H2O | - |
Fusarium solani | p-nitrophenol + butyrate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
ANS binds strongly to native cutinase as a noncompetitive inhibitor with up to 5 ANS per cutinase molecule. The first ANS molecule stabilizes cutinase. The last 4 ANS molecules decrease Tm-value by up to 7°C | Fusarium solani |
40 | - |
0.08 mM cutinase, pH 8.0, no additive, Tm-value is 40.3°C | Fusarium solani |
48 | - |
0.08 mM cutinase or 0.005 mM cutinase, pH 8.0, 2 M urea, Tm-value 47.5°C | Fusarium solani |
52 | - |
0.06 mM cutinase, pH 8.0, Tm-value is 52.0°C | Fusarium solani |
55 | - |
0.08 mM cutinase, pH 8.0, 20 mM 0.5 M L-Arg, Tm-value | Fusarium solani |
56 | - |
0.005 mM cutinase, pH 8.0, 20 mM 0.5 M L-Arg, Tm-value is 56.4°C | Fusarium solani |
57 | - |
0.04 mM cutinase, pH 8.0, Tm-value is 57.3°C | Fusarium solani |
59 | - |
0.0001 mM cutinase, pH 8.0, Tm-value is 59.3°C | Fusarium solani |
59 | - |
0.005 mM cutinase, pH 8.0, Tm-value is 59.3°C | Fusarium solani |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Fusarium solani | - |
- |
8 |