Literature summary for 3.1.1.74 extracted from

Purdy, R.E.; Kolattukudy, P.E.
Hydrolysis of plant cuticle by plant pathogens. Properties of cutinase I, cutinase II, and a nonspecific esterase isolated from Fusarium solani pisi (1975), Biochemistry, 14, 2832-2840.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallization of native enzyme, 27 mutant enzymes and 4 covalently inhibited complexes, crystallizes in 8 different crystal forms	Fusarium solani

Inhibitors

Inhibitors	Comment	Organism	Structure
diisopropyl fluorophosphate	-	Fusarium solani
paraoxon	-	Fusarium solani

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.35	-	p-nitrophenylbutanoate	cutinase I	Fusarium solani
0.36	-	p-nitrophenyldecanoate	cutinase II	Fusarium solani
0.45	-	p-nitrophenyldodecanoate	cutinase II	Fusarium solani
0.48	-	p-nitrophenyldecanoate	cutinase I	Fusarium solani
0.56	-	p-nitrophenyldodecanoate	cutinase I	Fusarium solani
0.59	-	4-nitrophenyl octanoate	cutinase II	Fusarium solani
0.75	-	p-nitrophenylbutanoate	cutinase II	Fusarium solani
0.86	-	4-nitrophenyl hexanoate	cutinase II	Fusarium solani
0.88	-	4-nitrophenyl octanoate	cutinase I	Fusarium solani
0.89	-	4-nitrophenyl hexanoate	cutinase I	Fusarium solani
6.8	-	4-nitrophenyl acetate	cutinase I	Fusarium solani
9.7	-	4-nitrophenyl acetate	cutinase II	Fusarium solani

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Fusarium solani	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20400	-	cutinase II, equilibrium sedimentation	Fusarium solani
21400	-	1 * 21400, cutinase I and II, SDS-PAGE	Fusarium solani
21800	-	cutinase I and II, gel filtration	Fusarium solani
23400	-	cutinase I, equilibrium sedimentation	Fusarium solani

Organism

Organism	UniProt	Comment	Textmining
Fusarium solani	-	cutinase I and cutinase II	-
Fusarium solani	-	pisi	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture medium	-	Fusarium solani	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	Fusarium solani	4-nitrophenol + acetate	-	?
4-nitrophenyl dodecanoate + H2O	-	Fusarium solani	4-nitrophenol + dodecanoate	-	?
4-nitrophenyl octanoate + H2O	-	Fusarium solani	4-nitrophenol + octanoate	-	?
cutin + H2O	-	Fusarium solani	additional information	dihydroxyhexadecanoic acid, cutin monomer + cutin oligomers	?
cyclohexyl hexadecanoate + H2O	-	Fusarium solani	hexadecanoic acid + cyclohexanol	-	?
hexadecyl hexadecanoate + H2O	weak activity	Fusarium solani	hexadecanoic acid + hexadecanol	-	?
methyl hexadecanoate + H2O	-	Fusarium solani	hexadecanoic acid + methanol	-	?
p-nitrophenyl acetate + H2O	-	Fusarium solani	p-nitrophenol + acetate	-	?
p-nitrophenyl hexanoate + H2O	-	Fusarium solani	p-nitrophenol + hexanoate	-	?
p-nitrophenylbutanoate + H2O	-	Fusarium solani	p-nitrophenol + butanoate	-	?
p-nitrophenyldecanoate + H2O	-	Fusarium solani	p-nitrophenol + decanoate	-	?

Subunits

Subunits	Comment	Organism
?	x * 21800, cutinase I, SDS-PAGE	Fusarium solani
?	x * 21800, and a smaller band of 10600 Da, cutinase II, SDS-PAGE	Fusarium solani
monomer	1 * 21400, cutinase I and II, SDS-PAGE	Fusarium solani

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
10	-	-	Fusarium solani

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Release 2023.1 (January 2023)